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Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection

BACKGROUND: Malaria remains a serious threat to global public health. With poor efficacies of vaccines and the emergence of drug resistance, novel strategies to control malaria are urgently needed. RESULTS: We developed erythrocyte membrane-camouflaged nanoparticles loaded with artemether based on t...

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Autores principales: He, Zhouqing, Yu, Chuyi, Pan, Ziyi, Li, Xiaobo, Zhang, Xiangxiang, Huang, Qijing, Liao, Xingcheng, Hu, Jiaoting, Zeng, Feng, Ru, Li, Yu, Wanlin, Xu, Qin, Song, Jianping, Liang, Jianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841648/
https://www.ncbi.nlm.nih.gov/pubmed/36647056
http://dx.doi.org/10.1186/s12951-022-01709-x
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author He, Zhouqing
Yu, Chuyi
Pan, Ziyi
Li, Xiaobo
Zhang, Xiangxiang
Huang, Qijing
Liao, Xingcheng
Hu, Jiaoting
Zeng, Feng
Ru, Li
Yu, Wanlin
Xu, Qin
Song, Jianping
Liang, Jianming
author_facet He, Zhouqing
Yu, Chuyi
Pan, Ziyi
Li, Xiaobo
Zhang, Xiangxiang
Huang, Qijing
Liao, Xingcheng
Hu, Jiaoting
Zeng, Feng
Ru, Li
Yu, Wanlin
Xu, Qin
Song, Jianping
Liang, Jianming
author_sort He, Zhouqing
collection PubMed
description BACKGROUND: Malaria remains a serious threat to global public health. With poor efficacies of vaccines and the emergence of drug resistance, novel strategies to control malaria are urgently needed. RESULTS: We developed erythrocyte membrane-camouflaged nanoparticles loaded with artemether based on the growth characteristics of Plasmodium. The nanoparticles could capture the merozoites to inhibit them from repeatedly infecting normal erythrocytes, owing to the interactions between merozoites and heparin-like molecules on the erythrocyte membrane. Modification with a phosphatidylserine-targeting peptide (CLIPPKF) improved the drug accumulation in infected red blood cells (iRBCs) from the externalized phosphatidylserine induced by Plasmodium infection. In Plasmodium berghei ANKA strain (pbANKA)-infected C57BL/6 mice, the nanoparticles significantly attenuated Plasmodium-induced inflammation, apoptosis, and anemia. We observed reduced weight variation and prolonged survival time in pbANKA-challenged mice, and the nanoparticles showed good biocompatibility and negligible cytotoxicity. CONCLUSION: Erythrocyte membrane-camouflaged nanoparticles loaded with artemether were shown to provide safe and effective protection against Plasmodium infection. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01709-x.
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spelling pubmed-98416482023-01-17 Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection He, Zhouqing Yu, Chuyi Pan, Ziyi Li, Xiaobo Zhang, Xiangxiang Huang, Qijing Liao, Xingcheng Hu, Jiaoting Zeng, Feng Ru, Li Yu, Wanlin Xu, Qin Song, Jianping Liang, Jianming J Nanobiotechnology Research BACKGROUND: Malaria remains a serious threat to global public health. With poor efficacies of vaccines and the emergence of drug resistance, novel strategies to control malaria are urgently needed. RESULTS: We developed erythrocyte membrane-camouflaged nanoparticles loaded with artemether based on the growth characteristics of Plasmodium. The nanoparticles could capture the merozoites to inhibit them from repeatedly infecting normal erythrocytes, owing to the interactions between merozoites and heparin-like molecules on the erythrocyte membrane. Modification with a phosphatidylserine-targeting peptide (CLIPPKF) improved the drug accumulation in infected red blood cells (iRBCs) from the externalized phosphatidylserine induced by Plasmodium infection. In Plasmodium berghei ANKA strain (pbANKA)-infected C57BL/6 mice, the nanoparticles significantly attenuated Plasmodium-induced inflammation, apoptosis, and anemia. We observed reduced weight variation and prolonged survival time in pbANKA-challenged mice, and the nanoparticles showed good biocompatibility and negligible cytotoxicity. CONCLUSION: Erythrocyte membrane-camouflaged nanoparticles loaded with artemether were shown to provide safe and effective protection against Plasmodium infection. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01709-x. BioMed Central 2023-01-16 /pmc/articles/PMC9841648/ /pubmed/36647056 http://dx.doi.org/10.1186/s12951-022-01709-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
He, Zhouqing
Yu, Chuyi
Pan, Ziyi
Li, Xiaobo
Zhang, Xiangxiang
Huang, Qijing
Liao, Xingcheng
Hu, Jiaoting
Zeng, Feng
Ru, Li
Yu, Wanlin
Xu, Qin
Song, Jianping
Liang, Jianming
Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title_full Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title_fullStr Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title_full_unstemmed Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title_short Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection
title_sort erythrocyte membrane with clippkf as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent plasmodium infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841648/
https://www.ncbi.nlm.nih.gov/pubmed/36647056
http://dx.doi.org/10.1186/s12951-022-01709-x
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