Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

BACKGROUND: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. METHODOLOGY: To v...

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Detalles Bibliográficos
Autores principales: Martins Balbinot, Karolyny, Almeida Loureiro, Felippe José, Chemelo, Giordanna Pereira, Alves Mesquita, Ricardo, Cruz Ramos, Aline Maria Pereira, Ramos, Rommel Thiago Jucá, da Costa da Silva, Artur Luiz, de Menezes, Sílvio Augusto Fernandes, da Silva Kataoka, Maria Sueli, Alves Junior, Sergio de Melo, Viana Pinheiro, João de Jesus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841912/
https://www.ncbi.nlm.nih.gov/pubmed/36655039
http://dx.doi.org/10.7717/peerj.14349
Descripción
Sumario:BACKGROUND: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. METHODOLOGY: To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. RESULTS: Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). CONCLUSIONS: The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.

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