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Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma
BACKGROUND: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. METHODOLOGY: To v...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841912/ https://www.ncbi.nlm.nih.gov/pubmed/36655039 http://dx.doi.org/10.7717/peerj.14349 |
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author | Martins Balbinot, Karolyny Almeida Loureiro, Felippe José Chemelo, Giordanna Pereira Alves Mesquita, Ricardo Cruz Ramos, Aline Maria Pereira Ramos, Rommel Thiago Jucá da Costa da Silva, Artur Luiz de Menezes, Sílvio Augusto Fernandes da Silva Kataoka, Maria Sueli Alves Junior, Sergio de Melo Viana Pinheiro, João de Jesus |
author_facet | Martins Balbinot, Karolyny Almeida Loureiro, Felippe José Chemelo, Giordanna Pereira Alves Mesquita, Ricardo Cruz Ramos, Aline Maria Pereira Ramos, Rommel Thiago Jucá da Costa da Silva, Artur Luiz de Menezes, Sílvio Augusto Fernandes da Silva Kataoka, Maria Sueli Alves Junior, Sergio de Melo Viana Pinheiro, João de Jesus |
author_sort | Martins Balbinot, Karolyny |
collection | PubMed |
description | BACKGROUND: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. METHODOLOGY: To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. RESULTS: Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). CONCLUSIONS: The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME. |
format | Online Article Text |
id | pubmed-9841912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98419122023-01-17 Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma Martins Balbinot, Karolyny Almeida Loureiro, Felippe José Chemelo, Giordanna Pereira Alves Mesquita, Ricardo Cruz Ramos, Aline Maria Pereira Ramos, Rommel Thiago Jucá da Costa da Silva, Artur Luiz de Menezes, Sílvio Augusto Fernandes da Silva Kataoka, Maria Sueli Alves Junior, Sergio de Melo Viana Pinheiro, João de Jesus PeerJ Biochemistry BACKGROUND: Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. METHODOLOGY: To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. RESULTS: Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). CONCLUSIONS: The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME. PeerJ Inc. 2023-01-13 /pmc/articles/PMC9841912/ /pubmed/36655039 http://dx.doi.org/10.7717/peerj.14349 Text en © 2023 Martins Balbinot et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Martins Balbinot, Karolyny Almeida Loureiro, Felippe José Chemelo, Giordanna Pereira Alves Mesquita, Ricardo Cruz Ramos, Aline Maria Pereira Ramos, Rommel Thiago Jucá da Costa da Silva, Artur Luiz de Menezes, Sílvio Augusto Fernandes da Silva Kataoka, Maria Sueli Alves Junior, Sergio de Melo Viana Pinheiro, João de Jesus Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title | Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title_full | Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title_fullStr | Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title_full_unstemmed | Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title_short | Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma |
title_sort | immunoexpression of stem cell markers sox-2, nanog and oct4 in ameloblastoma |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841912/ https://www.ncbi.nlm.nih.gov/pubmed/36655039 http://dx.doi.org/10.7717/peerj.14349 |
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