Cargando…
Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR
Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841932/ https://www.ncbi.nlm.nih.gov/pubmed/36645640 http://dx.doi.org/10.1007/s42770-023-00902-w |
_version_ | 1784870006794747904 |
---|---|
author | Sales, Mariana Lázaro Fonseca Júnior, Antônio Augusto Pinheiro de Oliveira, Tatiana Flávia de Araújo Lopes, Amanda Rivetti Júnior, Anselmo Vasconcelos Camargos, Marcelo Fernandes dos Reis, Jenner Karlisson Pimenta |
author_facet | Sales, Mariana Lázaro Fonseca Júnior, Antônio Augusto Pinheiro de Oliveira, Tatiana Flávia de Araújo Lopes, Amanda Rivetti Júnior, Anselmo Vasconcelos Camargos, Marcelo Fernandes dos Reis, Jenner Karlisson Pimenta |
author_sort | Sales, Mariana Lázaro |
collection | PubMed |
description | Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study. |
format | Online Article Text |
id | pubmed-9841932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-98419322023-01-17 Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR Sales, Mariana Lázaro Fonseca Júnior, Antônio Augusto Pinheiro de Oliveira, Tatiana Flávia de Araújo Lopes, Amanda Rivetti Júnior, Anselmo Vasconcelos Camargos, Marcelo Fernandes dos Reis, Jenner Karlisson Pimenta Braz J Microbiol Veterinary Microbiology - Research Paper Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study. Springer International Publishing 2023-01-16 /pmc/articles/PMC9841932/ /pubmed/36645640 http://dx.doi.org/10.1007/s42770-023-00902-w Text en © The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
spellingShingle | Veterinary Microbiology - Research Paper Sales, Mariana Lázaro Fonseca Júnior, Antônio Augusto Pinheiro de Oliveira, Tatiana Flávia de Araújo Lopes, Amanda Rivetti Júnior, Anselmo Vasconcelos Camargos, Marcelo Fernandes dos Reis, Jenner Karlisson Pimenta Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title_full | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title_fullStr | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title_full_unstemmed | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title_short | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR |
title_sort | quantification of in vitro replication kinetics of alagoas vesiculovirus isolates by digital droplet rt-pcr |
topic | Veterinary Microbiology - Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841932/ https://www.ncbi.nlm.nih.gov/pubmed/36645640 http://dx.doi.org/10.1007/s42770-023-00902-w |
work_keys_str_mv | AT salesmarianalazaro quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT fonsecajuniorantonioaugusto quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT pinheirodeoliveiratatianaflavia quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT dearaujolopesamanda quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT rivettijunioranselmovasconcelos quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT camargosmarcelofernandes quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr AT dosreisjennerkarlissonpimenta quantificationofinvitroreplicationkineticsofalagoasvesiculovirusisolatesbydigitaldropletrtpcr |