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In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome

Antimicrobial resistance (AMR) is a global threat, with evolution and spread of resistance to frontline antibiotics outpacing the development of novel treatments. The spread of AMR is perpetuated by transfer of antimicrobial resistance genes (ARGs) between bacteria, notably those encoded by conjugat...

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Autores principales: Kessler, Celia, Hou, Jingping, Neo, Onalenna, Buckner, Michelle M C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841969/
https://www.ncbi.nlm.nih.gov/pubmed/36341518
http://dx.doi.org/10.1093/femsre/fuac044
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author Kessler, Celia
Hou, Jingping
Neo, Onalenna
Buckner, Michelle M C
author_facet Kessler, Celia
Hou, Jingping
Neo, Onalenna
Buckner, Michelle M C
author_sort Kessler, Celia
collection PubMed
description Antimicrobial resistance (AMR) is a global threat, with evolution and spread of resistance to frontline antibiotics outpacing the development of novel treatments. The spread of AMR is perpetuated by transfer of antimicrobial resistance genes (ARGs) between bacteria, notably those encoded by conjugative plasmids. The human gut microbiome is a known ‘melting pot’ for plasmid conjugation, with ARG transfer in this environment widely documented. There is a need to better understand the factors affecting the incidence of these transfer events, and to investigate methods of potentially counteracting the spread of ARGs. This review describes the use and potential of three approaches to studying conjugation in the human gut: observation of in situ events in hospitalized patients, modelling of the microbiome in vivo predominantly in rodent models, and the use of in vitro models of various complexities. Each has brought unique insights to our understanding of conjugation in the gut. The use and development of these systems, and combinations thereof, will be pivotal in better understanding the significance, prevalence, and manipulability of horizontal gene transfer in the gut microbiome.
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spelling pubmed-98419692023-01-19 In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome Kessler, Celia Hou, Jingping Neo, Onalenna Buckner, Michelle M C FEMS Microbiol Rev Review Article Antimicrobial resistance (AMR) is a global threat, with evolution and spread of resistance to frontline antibiotics outpacing the development of novel treatments. The spread of AMR is perpetuated by transfer of antimicrobial resistance genes (ARGs) between bacteria, notably those encoded by conjugative plasmids. The human gut microbiome is a known ‘melting pot’ for plasmid conjugation, with ARG transfer in this environment widely documented. There is a need to better understand the factors affecting the incidence of these transfer events, and to investigate methods of potentially counteracting the spread of ARGs. This review describes the use and potential of three approaches to studying conjugation in the human gut: observation of in situ events in hospitalized patients, modelling of the microbiome in vivo predominantly in rodent models, and the use of in vitro models of various complexities. Each has brought unique insights to our understanding of conjugation in the gut. The use and development of these systems, and combinations thereof, will be pivotal in better understanding the significance, prevalence, and manipulability of horizontal gene transfer in the gut microbiome. Oxford University Press 2022-11-07 /pmc/articles/PMC9841969/ /pubmed/36341518 http://dx.doi.org/10.1093/femsre/fuac044 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Kessler, Celia
Hou, Jingping
Neo, Onalenna
Buckner, Michelle M C
In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title_full In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title_fullStr In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title_full_unstemmed In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title_short In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome
title_sort in situ, in vivo, and in vitro approaches for studying amr plasmid conjugation in the gut microbiome
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841969/
https://www.ncbi.nlm.nih.gov/pubmed/36341518
http://dx.doi.org/10.1093/femsre/fuac044
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