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Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions

Adhesions are dense, fibrous bridges that adjoin tissue surfaces due to uncontrolled inflammation following postoperative mesothelial injury. A widely used adhesion barrier material in Seprafilm often fails to prevent transverse scar tissue deposition because of its poor mechanical properties, rapid...

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Autores principales: Erdi, Metecan, Rozyyev, Selim, Balabhadrapatruni, Manogna, Saruwatari, Michele S., Daristotle, John L., Ayyub, Omar B., Sandler, Anthony D., Kofinas, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842025/
https://www.ncbi.nlm.nih.gov/pubmed/36684071
http://dx.doi.org/10.1002/btm2.10335
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author Erdi, Metecan
Rozyyev, Selim
Balabhadrapatruni, Manogna
Saruwatari, Michele S.
Daristotle, John L.
Ayyub, Omar B.
Sandler, Anthony D.
Kofinas, Peter
author_facet Erdi, Metecan
Rozyyev, Selim
Balabhadrapatruni, Manogna
Saruwatari, Michele S.
Daristotle, John L.
Ayyub, Omar B.
Sandler, Anthony D.
Kofinas, Peter
author_sort Erdi, Metecan
collection PubMed
description Adhesions are dense, fibrous bridges that adjoin tissue surfaces due to uncontrolled inflammation following postoperative mesothelial injury. A widely used adhesion barrier material in Seprafilm often fails to prevent transverse scar tissue deposition because of its poor mechanical properties, rapid degradation profile, and difficulty in precise application. Solution blow spinning (SBS), a polymer fiber deposition technique, allows for the placement of in situ tissue‐conforming and tissue‐adherent scaffolds with exceptional mechanical properties. While biodegradable polymers such as poly(lactic‐co‐glycolic acid) (PLGA) have desirable strength, they exhibit bulk biodegradation rates and inflammatory profiles that limit their use as adhesion barriers and result in poor tissue adhesion. Here, viscoelastic poly(lactide‐co‐caprolactone) (PLCL) is used for its pertinent biodegradation mechanism. Because it degrades via surface erosion, spray deposited PLCL fibers can dissolve new connections formed by inflamed tissue, allowing them to function as an effective, durable, and easy‐to‐apply adhesion barrier. Degradation kinetics are tuned to match adhesion formation through the design of PLCL blends comprised of highly adhesive “low”‐molecular weight (LMW) constituents in a mechanically robust “high”‐molecular weight (HMW) matrix. In vitro studies demonstrate that blending LMW PLCL (30% w/v) with HMW PLCL (70% w/v) yields an anti‐fibrotic yet tissue‐adhesive polymer sealant with a 14‐day erosion rate countering adhesion formation. PLCL blends additionally exhibit improved wet tissue adhesion strength (~10 kPa) over a 14‐day period versus previously explored biodegradable polymer compositions, such as PLGA. In a mouse cecal ligation model, select PLCL blends significantly reduce abdominal adhesions severity versus no treatment and Seprafilm‐treated controls.
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spelling pubmed-98420252023-01-19 Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions Erdi, Metecan Rozyyev, Selim Balabhadrapatruni, Manogna Saruwatari, Michele S. Daristotle, John L. Ayyub, Omar B. Sandler, Anthony D. Kofinas, Peter Bioeng Transl Med Research Articles Adhesions are dense, fibrous bridges that adjoin tissue surfaces due to uncontrolled inflammation following postoperative mesothelial injury. A widely used adhesion barrier material in Seprafilm often fails to prevent transverse scar tissue deposition because of its poor mechanical properties, rapid degradation profile, and difficulty in precise application. Solution blow spinning (SBS), a polymer fiber deposition technique, allows for the placement of in situ tissue‐conforming and tissue‐adherent scaffolds with exceptional mechanical properties. While biodegradable polymers such as poly(lactic‐co‐glycolic acid) (PLGA) have desirable strength, they exhibit bulk biodegradation rates and inflammatory profiles that limit their use as adhesion barriers and result in poor tissue adhesion. Here, viscoelastic poly(lactide‐co‐caprolactone) (PLCL) is used for its pertinent biodegradation mechanism. Because it degrades via surface erosion, spray deposited PLCL fibers can dissolve new connections formed by inflamed tissue, allowing them to function as an effective, durable, and easy‐to‐apply adhesion barrier. Degradation kinetics are tuned to match adhesion formation through the design of PLCL blends comprised of highly adhesive “low”‐molecular weight (LMW) constituents in a mechanically robust “high”‐molecular weight (HMW) matrix. In vitro studies demonstrate that blending LMW PLCL (30% w/v) with HMW PLCL (70% w/v) yields an anti‐fibrotic yet tissue‐adhesive polymer sealant with a 14‐day erosion rate countering adhesion formation. PLCL blends additionally exhibit improved wet tissue adhesion strength (~10 kPa) over a 14‐day period versus previously explored biodegradable polymer compositions, such as PLGA. In a mouse cecal ligation model, select PLCL blends significantly reduce abdominal adhesions severity versus no treatment and Seprafilm‐treated controls. John Wiley & Sons, Inc. 2022-05-23 /pmc/articles/PMC9842025/ /pubmed/36684071 http://dx.doi.org/10.1002/btm2.10335 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Erdi, Metecan
Rozyyev, Selim
Balabhadrapatruni, Manogna
Saruwatari, Michele S.
Daristotle, John L.
Ayyub, Omar B.
Sandler, Anthony D.
Kofinas, Peter
Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title_full Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title_fullStr Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title_full_unstemmed Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title_short Sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
title_sort sprayable tissue adhesive with biodegradation tuned for prevention of postoperative abdominal adhesions
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842025/
https://www.ncbi.nlm.nih.gov/pubmed/36684071
http://dx.doi.org/10.1002/btm2.10335
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