Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration

Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were p...

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Autores principales: Jiang, Zhiwei, Li, Na, Shao, Qin, Zhu, Danji, Feng, Yuting, Wang, Yang, Yu, Mengjia, Ren, Lingfei, Chen, Qianming, Yang, Guoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842060/
https://www.ncbi.nlm.nih.gov/pubmed/36684075
http://dx.doi.org/10.1002/btm2.10334
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author Jiang, Zhiwei
Li, Na
Shao, Qin
Zhu, Danji
Feng, Yuting
Wang, Yang
Yu, Mengjia
Ren, Lingfei
Chen, Qianming
Yang, Guoli
author_facet Jiang, Zhiwei
Li, Na
Shao, Qin
Zhu, Danji
Feng, Yuting
Wang, Yang
Yu, Mengjia
Ren, Lingfei
Chen, Qianming
Yang, Guoli
author_sort Jiang, Zhiwei
collection PubMed
description Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self‐renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold‐free 3D aggregates using light‐controlled cell sheet technology and a serum‐free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate‐implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.
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spelling pubmed-98420602023-01-19 Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration Jiang, Zhiwei Li, Na Shao, Qin Zhu, Danji Feng, Yuting Wang, Yang Yu, Mengjia Ren, Lingfei Chen, Qianming Yang, Guoli Bioeng Transl Med Research Articles Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self‐renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold‐free 3D aggregates using light‐controlled cell sheet technology and a serum‐free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate‐implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine. John Wiley & Sons, Inc. 2022-05-13 /pmc/articles/PMC9842060/ /pubmed/36684075 http://dx.doi.org/10.1002/btm2.10334 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Jiang, Zhiwei
Li, Na
Shao, Qin
Zhu, Danji
Feng, Yuting
Wang, Yang
Yu, Mengjia
Ren, Lingfei
Chen, Qianming
Yang, Guoli
Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title_full Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title_fullStr Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title_full_unstemmed Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title_short Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
title_sort light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842060/
https://www.ncbi.nlm.nih.gov/pubmed/36684075
http://dx.doi.org/10.1002/btm2.10334
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