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Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts
Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pan...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842613/ https://www.ncbi.nlm.nih.gov/pubmed/36646780 http://dx.doi.org/10.1038/s41598-023-27810-1 |
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author | Vigan-Womas, Inès Spadoni, Jean-Louis Poiret, Thomas Taïeb, Fabien Randrianarisaona, Fanirisoa Faye, Rokhaya Mbow, Adji Astou Gaye, Aboubacry Dia, Ndongo Loucoubar, Cheikh Ny Mioramalala, Diary Juliannie Ratovoson, Rila Randremanana, Rindra Vatosoa Sall, Amadou Alpha Seydi, Moussa Noirel, Josselin Moreau, Gabriel Simon, Arnaud Holenya, Pavlo Meyniel, Jean-Philippe Zagury, Jean-François Schoenhals, Matthieu |
author_facet | Vigan-Womas, Inès Spadoni, Jean-Louis Poiret, Thomas Taïeb, Fabien Randrianarisaona, Fanirisoa Faye, Rokhaya Mbow, Adji Astou Gaye, Aboubacry Dia, Ndongo Loucoubar, Cheikh Ny Mioramalala, Diary Juliannie Ratovoson, Rila Randremanana, Rindra Vatosoa Sall, Amadou Alpha Seydi, Moussa Noirel, Josselin Moreau, Gabriel Simon, Arnaud Holenya, Pavlo Meyniel, Jean-Philippe Zagury, Jean-François Schoenhals, Matthieu |
author_sort | Vigan-Womas, Inès |
collection | PubMed |
description | Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. |
format | Online Article Text |
id | pubmed-9842613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98426132023-01-18 Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts Vigan-Womas, Inès Spadoni, Jean-Louis Poiret, Thomas Taïeb, Fabien Randrianarisaona, Fanirisoa Faye, Rokhaya Mbow, Adji Astou Gaye, Aboubacry Dia, Ndongo Loucoubar, Cheikh Ny Mioramalala, Diary Juliannie Ratovoson, Rila Randremanana, Rindra Vatosoa Sall, Amadou Alpha Seydi, Moussa Noirel, Josselin Moreau, Gabriel Simon, Arnaud Holenya, Pavlo Meyniel, Jean-Philippe Zagury, Jean-François Schoenhals, Matthieu Sci Rep Article Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development. Nature Publishing Group UK 2023-01-16 /pmc/articles/PMC9842613/ /pubmed/36646780 http://dx.doi.org/10.1038/s41598-023-27810-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Vigan-Womas, Inès Spadoni, Jean-Louis Poiret, Thomas Taïeb, Fabien Randrianarisaona, Fanirisoa Faye, Rokhaya Mbow, Adji Astou Gaye, Aboubacry Dia, Ndongo Loucoubar, Cheikh Ny Mioramalala, Diary Juliannie Ratovoson, Rila Randremanana, Rindra Vatosoa Sall, Amadou Alpha Seydi, Moussa Noirel, Josselin Moreau, Gabriel Simon, Arnaud Holenya, Pavlo Meyniel, Jean-Philippe Zagury, Jean-François Schoenhals, Matthieu Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title | Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title_full | Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title_fullStr | Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title_full_unstemmed | Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title_short | Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts |
title_sort | linear epitope mapping of the humoral response against sars-cov-2 in two independent african cohorts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842613/ https://www.ncbi.nlm.nih.gov/pubmed/36646780 http://dx.doi.org/10.1038/s41598-023-27810-1 |
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