Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs

The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and...

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Autores principales: Ouyang, Maggie Jing, Ao, Zhujun, Olukitibi, Titus A., Yao, Xiao-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842621/
https://www.ncbi.nlm.nih.gov/pubmed/36853685
http://dx.doi.org/10.1016/j.xpro.2023.102083
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author Ouyang, Maggie Jing
Ao, Zhujun
Olukitibi, Titus A.
Yao, Xiao-Jian
author_facet Ouyang, Maggie Jing
Ao, Zhujun
Olukitibi, Titus A.
Yao, Xiao-Jian
author_sort Ouyang, Maggie Jing
collection PubMed
description The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and SARS-CoV-2-based virus-like particles and then evaluate the inflammatory cytokines’ protein and mRNA levels produced in human macrophages by S of SARS-CoV-2 original strain and Delta variant. This protocol is applicable in evaluating S from different emerging variants. For complete details on the use and execution of this protocol, please refer to Ao et al. (2022).(1)
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spelling pubmed-98426212023-01-17 Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs Ouyang, Maggie Jing Ao, Zhujun Olukitibi, Titus A. Yao, Xiao-Jian STAR Protoc Protocol The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and SARS-CoV-2-based virus-like particles and then evaluate the inflammatory cytokines’ protein and mRNA levels produced in human macrophages by S of SARS-CoV-2 original strain and Delta variant. This protocol is applicable in evaluating S from different emerging variants. For complete details on the use and execution of this protocol, please refer to Ao et al. (2022).(1) Elsevier 2023-01-17 /pmc/articles/PMC9842621/ /pubmed/36853685 http://dx.doi.org/10.1016/j.xpro.2023.102083 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ouyang, Maggie Jing
Ao, Zhujun
Olukitibi, Titus A.
Yao, Xiao-Jian
Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title_full Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title_fullStr Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title_full_unstemmed Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title_short Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs
title_sort protocol to evaluate the inflammatory response in human macrophages induced by sars-cov-2 spike-pseudotyped vlps
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842621/
https://www.ncbi.nlm.nih.gov/pubmed/36853685
http://dx.doi.org/10.1016/j.xpro.2023.102083
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