Optogenetic dephosphorylation of phosphatidylinositol 4,5 bisphosphate in Xenopus laevis oocytes

Here, we present a protocol for optogenetic dephosphorylation of the phosphoinositide PI(4,5)P(2) at the plasma membrane of Xenopus laevis oocytes. We first describe the co-injection of oocytes with cRNAs encoding (1) a light-activated PI(4,5)P(2) 5-phosphatase fusion protein, (2) its dimerization p...

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Detalles Bibliográficos
Autores principales: Gada, Kirin D., Xu, Yu, Winn, Brenda T., Masotti, Meghan, Kawano, Takeharu, Vaananen, Heikki, Plant, Leigh D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843251/
https://www.ncbi.nlm.nih.gov/pubmed/36633950
http://dx.doi.org/10.1016/j.xpro.2022.102003

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