Cargando…

In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for...

Descripción completa

Detalles Bibliográficos
Autores principales:	Safi, Fatemeh, Dhapola, Parashar, Erlandsson, Eva, Ulfsson, Linda Geironson, Calderón, Ariana S., Böiers, Charlotta, Karlsson, Göran
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843257/ https://www.ncbi.nlm.nih.gov/pubmed/36633951 http://dx.doi.org/10.1016/j.xpro.2022.101965

Ejemplares similares

Single-cell multiomics of human fetal hematopoiesis define a developmental-specific population and a fetal signature
por: Sommarin, Mikael N. E., et al.
Publicado: (2023)

Editorial: Fetal/Embryonic Hematopoietic Progenitors and Their Impact on Adult Diseases
por: Azzoni, Emanuele, et al.
Publicado: (2021)

lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation
por: Delás, M. Joaquina, et al.
Publicado: (2019)

Scarf enables a highly memory-efficient analysis of large-scale single-cell genomics data
por: Dhapola, Parashar, et al.
Publicado: (2022)

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells
por: Eisele, Almut S, et al.
Publicado: (2022)

Defining the Emerging Blood System During Development at Single-Cell Resolution
por: Karlsson, Göran, et al.
Publicado: (2021)

Corrigendum: Cocaine exposure impairs multilineage hematopoiesis of human hematopoietic progenitor cells mediated by the sigma-1 receptor
por: Nixon, Christopher C., et al.
Publicado: (2015)

Efficient long-term multilineage engraftment of CD33-edited hematopoietic stem/progenitor cells in nonhuman primates
por: Petty, Nicholas E., et al.
Publicado: (2023)

Identification of a Proximal Progenitor Population from Murine Fetal Lungs with Clonogenic and Multilineage Differentiation Potential
por: Bilodeau, Mélanie, et al.
Publicado: (2014)

Improved multilineage human hematopoietic reconstitution and function in NSGS mice
por: Wunderlich, Mark, et al.
Publicado: (2018)

Robust generation of erythroid and multilineage hematopoietic progenitors from human iPSCs using a scalable monolayer culture system
por: Ruiz, Juan Pablo, et al.
Publicado: (2019)

Autologous, Gene-Modified Hematopoietic Stem and Progenitor Cells Repopulate the Central Nervous System with Distinct Clonal Variants
por: Peterson, Christopher W., et al.
Publicado: (2019)

Dedifferentiation of Human Primary Thyrocytes into Multilineage Progenitor Cells without Gene Introduction
por: Suzuki, Keiji, et al.
Publicado: (2011)

Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity
por: Hara, Akitoshi, et al.
Publicado: (2021)

Chromatin accessibility maps provide evidence of multilineage gene priming in hematopoietic stem cells
por: Martin, Eric W., et al.
Publicado: (2021)

Tendon-Derived Progenitor Cells With Multilineage Potential Are Present Within Human Patellar Tendon
por: Leonardi, Erika A., et al.
Publicado: (2021)

Analysis of self-renewing and differentiation-related microRNAs and transcription factors in multilineage mouse hematopoietic stem/progenitor cells induced by 1,4-benzoquinone
por: Dewi, Ramya, et al.
Publicado: (2023)

Direct Reprogramming of Murine Fibroblasts to Hematopoietic Progenitor Cells
por: Batta, Kiran, et al.
Publicado: (2014)

Bcl11b mutations identified in murine lymphomas increase the proliferation rate of hematopoietic progenitor cells
por: Karlsson, Anneli, et al.
Publicado: (2007)

Multilineage differentiation potential of hematoendothelial progenitors derived from human induced pluripotent stem cells
por: Netsrithong, Ratchapong, et al.
Publicado: (2020)

Direct role of FLT3 in regulation of early lymphoid progenitors
por: Zriwil, Alya, et al.
Publicado: (2018)

Unbiased phenotypic identification of functionally distinct hematopoietic progenitors
por: Georgolopoulos, Grigorios, et al.
Publicado: (2019)

Sulfatase modifying factor 1–mediated fibroblast growth factor signaling primes hematopoietic multilineage development
por: Buono, Mario, et al.
Publicado: (2010)

RUNX1 deficiency cooperates with SRSF2 mutation to induce multilineage hematopoietic defects characteristic of MDS
por: Huang, Yi-Jou, et al.
Publicado: (2022)

Multilineage murine stem cells generate complex organoids to model distal lung development and disease
por: Vazquez‐Armendariz, Ana Ivonne, et al.
Publicado: (2020)

Clonal analysis reveals multiple functional defects of aged murine hematopoietic stem cells
por: Dykstra, Brad, et al.
Publicado: (2011)

Frequent reconstitution of IDH2(R140Q) mutant clonal multilineage hematopoiesis following chemotherapy for acute myeloid leukemia
por: Wiseman, Daniel H., et al.
Publicado: (2016)

Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term multilineage repopulating activity
por: Kataoka, Keisuke, et al.
Publicado: (2011)

Single low-dose rHuIL-12 safely triggers multilineage hematopoietic and immune-mediated effects
por: Gokhale, Mamata S, et al.
Publicado: (2014)

QuadBase2: web server for multiplexed guanine quadruplex mining and visualization
por: Dhapola, Parashar, et al.
Publicado: (2016)

Multilineage hematopoietic recovery with concomitant antitumor effects using low dose Interleukin-12 in myelosuppressed tumor-bearing mice
por: Basile, Lena A, et al.
Publicado: (2008)

Neuronal vulnerability and multilineage diversity in multiple sclerosis
por: Schirmer, Lucas, et al.
Publicado: (2019)

Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest‐derived multilineage cells
por: Abe, Shigehiro, et al.
Publicado: (2022)

Novel role of interleukin 7 in myelopoiesis: stimulation of primitive murine hematopoietic progenitor cells
Publicado: (1993)

Characterizing the Lymphopoietic Kinetics and Features of Hematopoietic Progenitors Contained in the Adult Murine Liver In Vivo
por: Jiang, Xiaojun, et al.
Publicado: (2013)

Proteinase 3 Limits the Number of Hematopoietic Stem and Progenitor Cells in Murine Bone Marrow
por: Karatepe, Kutay, et al.
Publicado: (2018)

Importance of Bcl-2-family proteins in murine hematopoietic progenitor and early B cells
por: Kurschat, Constanze, et al.
Publicado: (2021)

Gangliosides and Their Role in Multilineage Differentiation of Mesenchymal Stem Cells
por: Santilli, Francesca, et al.
Publicado: (2022)

BreCAN-DB: a repository cum browser of personalized DNA breakpoint profiles of cancer genomes
por: Narang, Pankaj, et al.
Publicado: (2016)

Schlafen2 is a regulator of quiescence in adult murine hematopoietic stem cells
por: Warsi, Sarah, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_1id5uflukj7rvhtr09e31mponr