A simplified courtship conditioning protocol to test learning and memory in Drosophila

In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for as...

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Detalles Bibliográficos
Autores principales: Gil-Martí, Beatriz, Barredo, Celia G., Pina-Flores, Sara, Poza-Rodriguez, Adriana, Treves, Gaia, Rodriguez-Navas, Carmen, Camacho, Lucía, Pérez-Serna, Atenea, Jimenez, Iñaki, Brazales, Laura, Fernandez, Javier, Martin, Francisco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843266/
https://www.ncbi.nlm.nih.gov/pubmed/36633946
http://dx.doi.org/10.1016/j.xpro.2022.101572
Descripción
Sumario:In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory.

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