A simplified courtship conditioning protocol to test learning and memory in Drosophila

In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for as...

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Autores principales: Gil-Martí, Beatriz, Barredo, Celia G., Pina-Flores, Sara, Poza-Rodriguez, Adriana, Treves, Gaia, Rodriguez-Navas, Carmen, Camacho, Lucía, Pérez-Serna, Atenea, Jimenez, Iñaki, Brazales, Laura, Fernandez, Javier, Martin, Francisco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843266/
https://www.ncbi.nlm.nih.gov/pubmed/36633946
http://dx.doi.org/10.1016/j.xpro.2022.101572
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author Gil-Martí, Beatriz
Barredo, Celia G.
Pina-Flores, Sara
Poza-Rodriguez, Adriana
Treves, Gaia
Rodriguez-Navas, Carmen
Camacho, Lucía
Pérez-Serna, Atenea
Jimenez, Iñaki
Brazales, Laura
Fernandez, Javier
Martin, Francisco A.
author_facet Gil-Martí, Beatriz
Barredo, Celia G.
Pina-Flores, Sara
Poza-Rodriguez, Adriana
Treves, Gaia
Rodriguez-Navas, Carmen
Camacho, Lucía
Pérez-Serna, Atenea
Jimenez, Iñaki
Brazales, Laura
Fernandez, Javier
Martin, Francisco A.
author_sort Gil-Martí, Beatriz
collection PubMed
description In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory.
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spelling pubmed-98432662023-01-18 A simplified courtship conditioning protocol to test learning and memory in Drosophila Gil-Martí, Beatriz Barredo, Celia G. Pina-Flores, Sara Poza-Rodriguez, Adriana Treves, Gaia Rodriguez-Navas, Carmen Camacho, Lucía Pérez-Serna, Atenea Jimenez, Iñaki Brazales, Laura Fernandez, Javier Martin, Francisco A. STAR Protoc Protocol In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory. Elsevier 2023-01-11 /pmc/articles/PMC9843266/ /pubmed/36633946 http://dx.doi.org/10.1016/j.xpro.2022.101572 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Gil-Martí, Beatriz
Barredo, Celia G.
Pina-Flores, Sara
Poza-Rodriguez, Adriana
Treves, Gaia
Rodriguez-Navas, Carmen
Camacho, Lucía
Pérez-Serna, Atenea
Jimenez, Iñaki
Brazales, Laura
Fernandez, Javier
Martin, Francisco A.
A simplified courtship conditioning protocol to test learning and memory in Drosophila
title A simplified courtship conditioning protocol to test learning and memory in Drosophila
title_full A simplified courtship conditioning protocol to test learning and memory in Drosophila
title_fullStr A simplified courtship conditioning protocol to test learning and memory in Drosophila
title_full_unstemmed A simplified courtship conditioning protocol to test learning and memory in Drosophila
title_short A simplified courtship conditioning protocol to test learning and memory in Drosophila
title_sort simplified courtship conditioning protocol to test learning and memory in drosophila
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843266/
https://www.ncbi.nlm.nih.gov/pubmed/36633946
http://dx.doi.org/10.1016/j.xpro.2022.101572
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