Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

A Gram-stain-negative, rod-shaped bacterial strain, JC4(T), was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4(T) is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133(T). The average nucleotide identity and digital DNA–DNA hybridization values between strain JC4(T) and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4(T) contained summed feature 3 (C(16:1) ω7c and/or C(16:1) ω6c) and iso-C(15:0) as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4(T) was determined to be 42.44%. The above polyphasic evidences support that strain JC4(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4(T) (= KCTC 92230(T) = LMG 32715(T)).