Cargando…

Development and validation of a duplex real-time PCR for the rapid detection and quantitation of HTLV-1

BACKGROUND: The HTLV-1 prevalence in China varies geographically, while HTLV-2 infection has rarely been found so far. Proviral load is one of the determining factors of pathogenesis and progression of HTLV-1 related diseases. However, neither molecular assays nor commercial kits are available for H...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ji, Huimin, Chang, Le, Yan, Ying, Wang, Lunan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843979/ https://www.ncbi.nlm.nih.gov/pubmed/36650537 http://dx.doi.org/10.1186/s12985-023-01970-y

Ejemplares similares

Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection
por: Sun, Shipeng, et al.
Publicado: (2011)

A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
por: Huang, Li, et al.
Publicado: (2015)

Development of real time PCR for detection and quantitation of Dengue Viruses
por: Gurukumar, KR, et al.
Publicado: (2009)

Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp
por: Leal, Carlos A G, et al.
Publicado: (2014)

Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR
por: Seaman, William T, et al.
Publicado: (2010)

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids
por: Yang, Litao, et al.
Publicado: (2008)

Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR
por: Chaturbedi, Abhishek, et al.
Publicado: (2012)

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
por: Divis, Paul CS, et al.
Publicado: (2010)

Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
por: Wong, Samson S. Y., et al.
Publicado: (2018)

Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products
por: Zheng, Weifeng, et al.
Publicado: (2019)

Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR
por: Zhang, Peng, et al.
Publicado: (2013)

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
por: Qiu, Fang-zhou, et al.
Publicado: (2018)

Development and Validation of Multiplex Quantitative Real-Time PCR Assays for Simultaneous Detection and Differentiation of HTLV-1 and HTLV-2, Using Different PCR Platforms and Reagent Brands
por: Gonçalves, Maria Gisele, et al.
Publicado: (2022)

Development of duplex PCR for differential detection of goatpox and sheeppox viruses
por: Zhao, Zhixun, et al.
Publicado: (2017)

Detection of North American orthopoxviruses by real time-PCR
por: Gallardo-Romero, Nadia F, et al.
Publicado: (2011)

Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus
por: Iwaki, Kosuke K, et al.
Publicado: (2010)

Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
por: Chen, Canbin, et al.
Publicado: (2019)

Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
por: Safeukui, Innocent, et al.
Publicado: (2008)

Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
por: Patel, Pranav, et al.
Publicado: (2013)

Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan
por: Rehman, Latif U, et al.
Publicado: (2011)

Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR
por: Li, Haiqin, et al.
Publicado: (2023)

Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
por: Verbrugghe, Phebe, et al.
Publicado: (2021)

Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients
por: Deschaght, Pieter, et al.
Publicado: (2009)

Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer
por: McNeill, Roisin E, et al.
Publicado: (2007)

Real-time PCR quantitation of glucocorticoid receptor alpha isoform
por: Melo, Murilo R, et al.
Publicado: (2004)

Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation
por: Faize, Mohamed, et al.
Publicado: (2010)

Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR
por: Strepparava, Nicole, et al.
Publicado: (2014)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
por: Su, Yi, et al.
Publicado: (2018)

Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR
por: Cnops, Lieselotte, et al.
Publicado: (2011)

A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences
por: Iraola, Gregorio, et al.
Publicado: (2016)

Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
por: Mucker, Eric M., et al.
Publicado: (2017)

Estimating the number of integrations in transformed plants by quantitative real-time PCR
por: Mason, Giovanna, et al.
Publicado: (2002)

Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil
por: Iotti, Mirco, et al.
Publicado: (2012)

Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
por: Ferreira, Isabel D, et al.
Publicado: (2006)

Development of Nested PCR and Duplex Real-Time Fluorescence Quantitative PCR Assay for the Simultaneous Detection of Theileria equi and Babesia caballi
por: Lv, Kunying, et al.
Publicado: (2022)

Development of real-time PCR for detection of Mycoplasma hominis
por: Baczynska, Agata, et al.
Publicado: (2004)

Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis
por: Espitia, Claudia M, et al.
Publicado: (2010)

Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
por: Hernández-Arteaga, Socorro, et al.
Publicado: (2010)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_f392f7hb631ub8sqegtgcafrh6