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Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4

BACKGROUND: Chemotherapy resistance is an important bottleneck affecting the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is of great significance for improving the prognosis of patients with colon cancer. METHODS: CCK-8 assay...

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Autores principales: Ying, Jun, Zhou, Haiyang, Wang, Zhiguo, You, Qing, Chen, Junnan, Lu, Hao, Zhang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843993/
https://www.ncbi.nlm.nih.gov/pubmed/36647071
http://dx.doi.org/10.1186/s12935-023-02847-4
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author Ying, Jun
Zhou, Haiyang
Wang, Zhiguo
You, Qing
Chen, Junnan
Lu, Hao
Zhang, Jian
author_facet Ying, Jun
Zhou, Haiyang
Wang, Zhiguo
You, Qing
Chen, Junnan
Lu, Hao
Zhang, Jian
author_sort Ying, Jun
collection PubMed
description BACKGROUND: Chemotherapy resistance is an important bottleneck affecting the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is of great significance for improving the prognosis of patients with colon cancer. METHODS: CCK-8 assay was employed to examine the cell viability of colorectal cancer cell lines. Realtime-PCR and western blot were used to explore toll-like receptor 4 (TLR4) expression in colorectal cancer cell lines. The functions of TLR4 in the stemness of the colorectal cancer cell lines were analyzed by infecting cells with lentivirus containing TLR4 siRNA. RESULTS: We found that aspirin could effectively enhance the chemosensitivity of CT26 and HCT116 colorectal cancer cell lines. Aspirin can also inhibit the stemness of colorectal cancer cell including inhibiting the number of clone formation and reducing the volume and number of cell spheres and inducing the down-regulation of stemness-related genes. Besides that, aspirin also lead to down-regulation of TLR4 expression in colorectal cancer cells. The TLR4 positive colorectal cancer cells demonstrated a higher chemotherapy resistance potential than TLR4 negative colorectal cancer cells. In addition, the stemness of TLR4 positive colorectal cancer cells is stronger than TLR4 negative colorectal cancer cells. CONCLUSION: The results of our study indicate that aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4.
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spelling pubmed-98439932023-01-18 Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4 Ying, Jun Zhou, Haiyang Wang, Zhiguo You, Qing Chen, Junnan Lu, Hao Zhang, Jian Cancer Cell Int Research BACKGROUND: Chemotherapy resistance is an important bottleneck affecting the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is of great significance for improving the prognosis of patients with colon cancer. METHODS: CCK-8 assay was employed to examine the cell viability of colorectal cancer cell lines. Realtime-PCR and western blot were used to explore toll-like receptor 4 (TLR4) expression in colorectal cancer cell lines. The functions of TLR4 in the stemness of the colorectal cancer cell lines were analyzed by infecting cells with lentivirus containing TLR4 siRNA. RESULTS: We found that aspirin could effectively enhance the chemosensitivity of CT26 and HCT116 colorectal cancer cell lines. Aspirin can also inhibit the stemness of colorectal cancer cell including inhibiting the number of clone formation and reducing the volume and number of cell spheres and inducing the down-regulation of stemness-related genes. Besides that, aspirin also lead to down-regulation of TLR4 expression in colorectal cancer cells. The TLR4 positive colorectal cancer cells demonstrated a higher chemotherapy resistance potential than TLR4 negative colorectal cancer cells. In addition, the stemness of TLR4 positive colorectal cancer cells is stronger than TLR4 negative colorectal cancer cells. CONCLUSION: The results of our study indicate that aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4. BioMed Central 2023-01-16 /pmc/articles/PMC9843993/ /pubmed/36647071 http://dx.doi.org/10.1186/s12935-023-02847-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ying, Jun
Zhou, Haiyang
Wang, Zhiguo
You, Qing
Chen, Junnan
Lu, Hao
Zhang, Jian
Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title_full Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title_fullStr Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title_full_unstemmed Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title_short Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
title_sort aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843993/
https://www.ncbi.nlm.nih.gov/pubmed/36647071
http://dx.doi.org/10.1186/s12935-023-02847-4
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