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Flow-Seq Method: Features and Application in Bacterial Translation Studies
The Flow-seq method is based on using reporter construct libraries, where a certain element regulating the gene expression of fluorescent reporter proteins is represented in many thousands of variants. Reporter construct libraries are introduced into cells, sorted according to their fluorescence lev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844084/ https://www.ncbi.nlm.nih.gov/pubmed/36694903 http://dx.doi.org/10.32607/actanaturae.11820 |
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author | Komarova, E. S. Dontsova, O. A. Pyshnyi, D. V. Kabilov, M. R. Sergiev, P. V. |
author_facet | Komarova, E. S. Dontsova, O. A. Pyshnyi, D. V. Kabilov, M. R. Sergiev, P. V. |
author_sort | Komarova, E. S. |
collection | PubMed |
description | The Flow-seq method is based on using reporter construct libraries, where a certain element regulating the gene expression of fluorescent reporter proteins is represented in many thousands of variants. Reporter construct libraries are introduced into cells, sorted according to their fluorescence level, and then subjected to next-generation sequencing. Therefore, it turns out to be possible to identify patterns that determine the expression efficiency, based on tens and hundreds of thousands of reporter constructs in one experiment. This method has become common in evaluating the efficiency of protein synthesis simultaneously by multiple mRNA variants. However, its potential is not confined to this area. In the presented review, a comparative analysis of the Flow-seq method and other alternative approaches used for translation efficiency evaluation of mRNA was carried out; the features of its application and the results obtained by Flow-seq were also considered. |
format | Online Article Text |
id | pubmed-9844084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-98440842023-01-23 Flow-Seq Method: Features and Application in Bacterial Translation Studies Komarova, E. S. Dontsova, O. A. Pyshnyi, D. V. Kabilov, M. R. Sergiev, P. V. Acta Naturae Research Article The Flow-seq method is based on using reporter construct libraries, where a certain element regulating the gene expression of fluorescent reporter proteins is represented in many thousands of variants. Reporter construct libraries are introduced into cells, sorted according to their fluorescence level, and then subjected to next-generation sequencing. Therefore, it turns out to be possible to identify patterns that determine the expression efficiency, based on tens and hundreds of thousands of reporter constructs in one experiment. This method has become common in evaluating the efficiency of protein synthesis simultaneously by multiple mRNA variants. However, its potential is not confined to this area. In the presented review, a comparative analysis of the Flow-seq method and other alternative approaches used for translation efficiency evaluation of mRNA was carried out; the features of its application and the results obtained by Flow-seq were also considered. A.I. Gordeyev 2022 /pmc/articles/PMC9844084/ /pubmed/36694903 http://dx.doi.org/10.32607/actanaturae.11820 Text en Copyright ® 2022 National Research University Higher School of Economics. https://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Komarova, E. S. Dontsova, O. A. Pyshnyi, D. V. Kabilov, M. R. Sergiev, P. V. Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title | Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title_full | Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title_fullStr | Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title_full_unstemmed | Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title_short | Flow-Seq Method: Features and Application in Bacterial Translation Studies |
title_sort | flow-seq method: features and application in bacterial translation studies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844084/ https://www.ncbi.nlm.nih.gov/pubmed/36694903 http://dx.doi.org/10.32607/actanaturae.11820 |
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