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Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering

3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, p...

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Detalles Bibliográficos
Autores principales: Züger, Fabian, Berner, Natascha, Gullo, Maurizio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844313/
https://www.ncbi.nlm.nih.gov/pubmed/36648813
http://dx.doi.org/10.3390/biomimetics8010027
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author Züger, Fabian
Berner, Natascha
Gullo, Maurizio R.
author_facet Züger, Fabian
Berner, Natascha
Gullo, Maurizio R.
author_sort Züger, Fabian
collection PubMed
description 3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, proliferation, differentiation and tissue maturation. This work introduces a cost-efficient gelatin-methylcellulose based hydrogel whose rheological properties can be independently optimized for optimal printability and tissue engineering. Hydrogel viscosities were designed to present three different temperature regimes: low viscosity for eased cell suspension and printing with minimal shear stress, form fidelity directly after printing and long term structural stability during incubation. Enzymatically crosslinked hydrogel scaffolds with stiffnesses ranging from 5 to 50 kPa were produced, enabling the hydrogel to biomimic cell environments for different types of tissues. The bioink showed high intrinsic cytocompatibility and tissues fabricated by embedding and bioprinting NIH 3T3 fibroblasts showed satisfactory viability. This novel hydrogel uses robust and inexpensive technology, which can be adjusted for implementation in tissue regeneration, e.g., in myocardial or neural tissue engineering.
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spelling pubmed-98443132023-01-18 Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering Züger, Fabian Berner, Natascha Gullo, Maurizio R. Biomimetics (Basel) Communication 3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, proliferation, differentiation and tissue maturation. This work introduces a cost-efficient gelatin-methylcellulose based hydrogel whose rheological properties can be independently optimized for optimal printability and tissue engineering. Hydrogel viscosities were designed to present three different temperature regimes: low viscosity for eased cell suspension and printing with minimal shear stress, form fidelity directly after printing and long term structural stability during incubation. Enzymatically crosslinked hydrogel scaffolds with stiffnesses ranging from 5 to 50 kPa were produced, enabling the hydrogel to biomimic cell environments for different types of tissues. The bioink showed high intrinsic cytocompatibility and tissues fabricated by embedding and bioprinting NIH 3T3 fibroblasts showed satisfactory viability. This novel hydrogel uses robust and inexpensive technology, which can be adjusted for implementation in tissue regeneration, e.g., in myocardial or neural tissue engineering. MDPI 2023-01-09 /pmc/articles/PMC9844313/ /pubmed/36648813 http://dx.doi.org/10.3390/biomimetics8010027 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Züger, Fabian
Berner, Natascha
Gullo, Maurizio R.
Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title_full Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title_fullStr Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title_full_unstemmed Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title_short Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
title_sort towards a novel cost-effective and versatile bioink for 3d-bioprinting in tissue engineering
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844313/
https://www.ncbi.nlm.nih.gov/pubmed/36648813
http://dx.doi.org/10.3390/biomimetics8010027
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