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Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering
3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, p...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844313/ https://www.ncbi.nlm.nih.gov/pubmed/36648813 http://dx.doi.org/10.3390/biomimetics8010027 |
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author | Züger, Fabian Berner, Natascha Gullo, Maurizio R. |
author_facet | Züger, Fabian Berner, Natascha Gullo, Maurizio R. |
author_sort | Züger, Fabian |
collection | PubMed |
description | 3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, proliferation, differentiation and tissue maturation. This work introduces a cost-efficient gelatin-methylcellulose based hydrogel whose rheological properties can be independently optimized for optimal printability and tissue engineering. Hydrogel viscosities were designed to present three different temperature regimes: low viscosity for eased cell suspension and printing with minimal shear stress, form fidelity directly after printing and long term structural stability during incubation. Enzymatically crosslinked hydrogel scaffolds with stiffnesses ranging from 5 to 50 kPa were produced, enabling the hydrogel to biomimic cell environments for different types of tissues. The bioink showed high intrinsic cytocompatibility and tissues fabricated by embedding and bioprinting NIH 3T3 fibroblasts showed satisfactory viability. This novel hydrogel uses robust and inexpensive technology, which can be adjusted for implementation in tissue regeneration, e.g., in myocardial or neural tissue engineering. |
format | Online Article Text |
id | pubmed-9844313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98443132023-01-18 Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering Züger, Fabian Berner, Natascha Gullo, Maurizio R. Biomimetics (Basel) Communication 3D-bioprinting for tissue regeneration relies on, among other things, hydrogels with favorable rheological properties. These include shear thinning for cell-friendly extrusion, post-printing structural stability as well as physiologically relevant elastic moduli needed for optimal cell attachment, proliferation, differentiation and tissue maturation. This work introduces a cost-efficient gelatin-methylcellulose based hydrogel whose rheological properties can be independently optimized for optimal printability and tissue engineering. Hydrogel viscosities were designed to present three different temperature regimes: low viscosity for eased cell suspension and printing with minimal shear stress, form fidelity directly after printing and long term structural stability during incubation. Enzymatically crosslinked hydrogel scaffolds with stiffnesses ranging from 5 to 50 kPa were produced, enabling the hydrogel to biomimic cell environments for different types of tissues. The bioink showed high intrinsic cytocompatibility and tissues fabricated by embedding and bioprinting NIH 3T3 fibroblasts showed satisfactory viability. This novel hydrogel uses robust and inexpensive technology, which can be adjusted for implementation in tissue regeneration, e.g., in myocardial or neural tissue engineering. MDPI 2023-01-09 /pmc/articles/PMC9844313/ /pubmed/36648813 http://dx.doi.org/10.3390/biomimetics8010027 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Züger, Fabian Berner, Natascha Gullo, Maurizio R. Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title | Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title_full | Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title_fullStr | Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title_full_unstemmed | Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title_short | Towards a Novel Cost-Effective and Versatile Bioink for 3D-Bioprinting in Tissue Engineering |
title_sort | towards a novel cost-effective and versatile bioink for 3d-bioprinting in tissue engineering |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844313/ https://www.ncbi.nlm.nih.gov/pubmed/36648813 http://dx.doi.org/10.3390/biomimetics8010027 |
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