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Measurement of Charge and Refractive Indices in Optically Trapped and Ionized Living Cells

The post-ionization dynamics of chemo-treated and untreated 4T1 breast cancer cells ionized by laser trapping techniques are studied. We have determined each cell’s charge and refractive index by developing a theoretical model for the forces determining the post-ionization dynamics. The shift in a c...

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Detalles Bibliográficos
Autores principales: Muhammed, Endris, Erenso, Daniel B., Gao, Ying, Chen, Li, Kelley, Michele, Vazquez, Carina, Gale, Mitchell, Nichols, Cody, Crogman, Horace T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844322/
https://www.ncbi.nlm.nih.gov/pubmed/36648994
http://dx.doi.org/10.3390/tomography9010007
Descripción
Sumario:The post-ionization dynamics of chemo-treated and untreated 4T1 breast cancer cells ionized by laser trapping techniques are studied. We have determined each cell’s charge and refractive index by developing a theoretical model for the forces determining the post-ionization dynamics. The shift in a cell’s refractive index due to an intense oscillating electric field was studied, and the results are reported here. We observed that a trapped cell, as it becomes charged, will eventually exit the trap perpendicular to the beam’s direction; this means that the electric force of the cell overcomes the trapping force. As a result, the cell’s conductivity changes due to the oscillating field, causing a decrease in the cell’s refractive index.