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Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster

RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously d...

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Autores principales: Tixtha, Erika J., Super, Meg K., Titus, M. Brandon, Bono, Jeremy M., Olesnicky, Eugenia C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844462/
https://www.ncbi.nlm.nih.gov/pubmed/36648904
http://dx.doi.org/10.3390/jdb11010002
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author Tixtha, Erika J.
Super, Meg K.
Titus, M. Brandon
Bono, Jeremy M.
Olesnicky, Eugenia C.
author_facet Tixtha, Erika J.
Super, Meg K.
Titus, M. Brandon
Bono, Jeremy M.
Olesnicky, Eugenia C.
author_sort Tixtha, Erika J.
collection PubMed
description RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the Drosophila larval peripheral system and in locomotor behavior. However, caper function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced caper function, through either a genetic lesion or RNA interference-mediated knockdown of caper in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. caper dysfunction also results in partial embryonic and larval lethality. Given that caper is highly conserved across metazoa, these findings may also be relevant to vertebrates.
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spelling pubmed-98444622023-01-18 Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster Tixtha, Erika J. Super, Meg K. Titus, M. Brandon Bono, Jeremy M. Olesnicky, Eugenia C. J Dev Biol Article RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the Drosophila larval peripheral system and in locomotor behavior. However, caper function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced caper function, through either a genetic lesion or RNA interference-mediated knockdown of caper in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. caper dysfunction also results in partial embryonic and larval lethality. Given that caper is highly conserved across metazoa, these findings may also be relevant to vertebrates. MDPI 2022-12-23 /pmc/articles/PMC9844462/ /pubmed/36648904 http://dx.doi.org/10.3390/jdb11010002 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tixtha, Erika J.
Super, Meg K.
Titus, M. Brandon
Bono, Jeremy M.
Olesnicky, Eugenia C.
Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title_full Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title_fullStr Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title_full_unstemmed Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title_short Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster
title_sort roles for the rna-binding protein caper in reproductive output in drosophila melanogaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844462/
https://www.ncbi.nlm.nih.gov/pubmed/36648904
http://dx.doi.org/10.3390/jdb11010002
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