A Robust Method for Perfusable Microvascular Network Formation In Vitro

Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key chara...

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Detalles Bibliográficos
Autores principales: Wan, Zhengpeng, Zhong, Amy X., Zhang, Shun, Pavlou, Georgios, Coughlin, Mark F., Shelton, Sarah E., Nguyen, Huu Tuan, Lorch, Jochen H., Barbie, David A., Kamm, Roger D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844969/
https://www.ncbi.nlm.nih.gov/pubmed/35373502
http://dx.doi.org/10.1002/smtd.202200143
Descripción
Sumario:Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.

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