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A Robust Method for Perfusable Microvascular Network Formation In Vitro
Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key chara...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844969/ https://www.ncbi.nlm.nih.gov/pubmed/35373502 http://dx.doi.org/10.1002/smtd.202200143 |
| _version_ | 1784870783956287488 |
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| author | Wan, Zhengpeng Zhong, Amy X. Zhang, Shun Pavlou, Georgios Coughlin, Mark F. Shelton, Sarah E. Nguyen, Huu Tuan Lorch, Jochen H. Barbie, David A. Kamm, Roger D. |
| author_facet | Wan, Zhengpeng Zhong, Amy X. Zhang, Shun Pavlou, Georgios Coughlin, Mark F. Shelton, Sarah E. Nguyen, Huu Tuan Lorch, Jochen H. Barbie, David A. Kamm, Roger D. |
| author_sort | Wan, Zhengpeng |
| collection | PubMed |
| description | Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established. |
| format | Online Article Text |
| id | pubmed-9844969 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-98449692023-01-17 A Robust Method for Perfusable Microvascular Network Formation In Vitro Wan, Zhengpeng Zhong, Amy X. Zhang, Shun Pavlou, Georgios Coughlin, Mark F. Shelton, Sarah E. Nguyen, Huu Tuan Lorch, Jochen H. Barbie, David A. Kamm, Roger D. Small Methods Article Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established. 2022-06 2022-04-03 /pmc/articles/PMC9844969/ /pubmed/35373502 http://dx.doi.org/10.1002/smtd.202200143 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
| spellingShingle | Article Wan, Zhengpeng Zhong, Amy X. Zhang, Shun Pavlou, Georgios Coughlin, Mark F. Shelton, Sarah E. Nguyen, Huu Tuan Lorch, Jochen H. Barbie, David A. Kamm, Roger D. A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_full | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_fullStr | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_full_unstemmed | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_short | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
| title_sort | robust method for perfusable microvascular network formation in vitro |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844969/ https://www.ncbi.nlm.nih.gov/pubmed/35373502 http://dx.doi.org/10.1002/smtd.202200143 |
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