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Identification of an unrecognized circRNA associated with development of renal fibrosis

Backgroud: Renal fibrosis is the common characteristic of chronic kidney disease. Circular RNA plays an essential role in the occurrence and development of Renal fibrosis, but its regulative mechanism remains elusive. Methods: The animal and cell model of Renal fibrosis was established, and RNA-sequ...

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Autores principales: Zhu, Yun, Yan, Weimin, Xu, Shuangyan, Yu, Xiaochao, Sun, Shuo, Zhang, Shaoxiong, Zhao, Ran, Tao, Jiayue, Li, Yunwei, Li, Cuie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845265/
https://www.ncbi.nlm.nih.gov/pubmed/36685959
http://dx.doi.org/10.3389/fgene.2022.964840
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author Zhu, Yun
Yan, Weimin
Xu, Shuangyan
Yu, Xiaochao
Sun, Shuo
Zhang, Shaoxiong
Zhao, Ran
Tao, Jiayue
Li, Yunwei
Li, Cuie
author_facet Zhu, Yun
Yan, Weimin
Xu, Shuangyan
Yu, Xiaochao
Sun, Shuo
Zhang, Shaoxiong
Zhao, Ran
Tao, Jiayue
Li, Yunwei
Li, Cuie
author_sort Zhu, Yun
collection PubMed
description Backgroud: Renal fibrosis is the common characteristic of chronic kidney disease. Circular RNA plays an essential role in the occurrence and development of Renal fibrosis, but its regulative mechanism remains elusive. Methods: The animal and cell model of Renal fibrosis was established, and RNA-sequencing and real-time polymerase chain reaction (qRT-PCR) experiments were implemented. Subsequently, experiments for detecting apoptosis and proliferation of cell, were carried out, and the isobaric tags for relative and absolute quantification proteomics analyses were performed accordingly. Results: It was found that a newly discovered Circular RNA (circRNA_0002158), is highly expressed in kidneys or cells with fibrosis, implying that this Circular RNA might be associated with the occurrence and development of Renal fibrosis. Subsequently, the overexpression and knockdown of circRNA_0002158 were conducted in the human kidney epithelial cell line (HK-2) cells, and the results indicated that the circRNA_0002158 could inhibit apoptosis, and promote proliferation of cells. The kidney injury-related factors, including Fibronectin and plasminogen activator inhibitor-1 (PAI-1), were decreased in HK-2 cells with overexpression of circRNA_0002158, while the results were reversed in cells with knockdown of circRNA_0002158. Finally, to explore the regulative mechanism of circRNA_0002158, the iTRAQ proteomics analyses were implemented for the cell samples with OE of circRNA_0002158 and its control, it showed that multiple genes and functional pathways were associated with the occurrence and development of Renal fibrosis. Conclusion: CircRNA_0002158 is associated with regulating Renal fibrosis, and may contribute to ameliorating the progression of Renal fibrosis in the future.
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spelling pubmed-98452652023-01-19 Identification of an unrecognized circRNA associated with development of renal fibrosis Zhu, Yun Yan, Weimin Xu, Shuangyan Yu, Xiaochao Sun, Shuo Zhang, Shaoxiong Zhao, Ran Tao, Jiayue Li, Yunwei Li, Cuie Front Genet Genetics Backgroud: Renal fibrosis is the common characteristic of chronic kidney disease. Circular RNA plays an essential role in the occurrence and development of Renal fibrosis, but its regulative mechanism remains elusive. Methods: The animal and cell model of Renal fibrosis was established, and RNA-sequencing and real-time polymerase chain reaction (qRT-PCR) experiments were implemented. Subsequently, experiments for detecting apoptosis and proliferation of cell, were carried out, and the isobaric tags for relative and absolute quantification proteomics analyses were performed accordingly. Results: It was found that a newly discovered Circular RNA (circRNA_0002158), is highly expressed in kidneys or cells with fibrosis, implying that this Circular RNA might be associated with the occurrence and development of Renal fibrosis. Subsequently, the overexpression and knockdown of circRNA_0002158 were conducted in the human kidney epithelial cell line (HK-2) cells, and the results indicated that the circRNA_0002158 could inhibit apoptosis, and promote proliferation of cells. The kidney injury-related factors, including Fibronectin and plasminogen activator inhibitor-1 (PAI-1), were decreased in HK-2 cells with overexpression of circRNA_0002158, while the results were reversed in cells with knockdown of circRNA_0002158. Finally, to explore the regulative mechanism of circRNA_0002158, the iTRAQ proteomics analyses were implemented for the cell samples with OE of circRNA_0002158 and its control, it showed that multiple genes and functional pathways were associated with the occurrence and development of Renal fibrosis. Conclusion: CircRNA_0002158 is associated with regulating Renal fibrosis, and may contribute to ameliorating the progression of Renal fibrosis in the future. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9845265/ /pubmed/36685959 http://dx.doi.org/10.3389/fgene.2022.964840 Text en Copyright © 2023 Zhu, Yan, Xu, Yu, Sun, Zhang, Zhao, Tao, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhu, Yun
Yan, Weimin
Xu, Shuangyan
Yu, Xiaochao
Sun, Shuo
Zhang, Shaoxiong
Zhao, Ran
Tao, Jiayue
Li, Yunwei
Li, Cuie
Identification of an unrecognized circRNA associated with development of renal fibrosis
title Identification of an unrecognized circRNA associated with development of renal fibrosis
title_full Identification of an unrecognized circRNA associated with development of renal fibrosis
title_fullStr Identification of an unrecognized circRNA associated with development of renal fibrosis
title_full_unstemmed Identification of an unrecognized circRNA associated with development of renal fibrosis
title_short Identification of an unrecognized circRNA associated with development of renal fibrosis
title_sort identification of an unrecognized circrna associated with development of renal fibrosis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845265/
https://www.ncbi.nlm.nih.gov/pubmed/36685959
http://dx.doi.org/10.3389/fgene.2022.964840
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