Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber

Pollen tube guidance within female tissues of flowering plants can be divided into preovular guidance, ovular guidance and a connecting stage called pollen tube emergence. As yet, no female factor has been identified to positively regulate this transition process. In this study, we show that an ovar...

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Autores principales: Cheng, Zhihua, Liu, Xiaofeng, Yan, Shuangshuang, Liu, Bin, Zhong, Yanting, Song, Weiyuan, Chen, Jiacai, Wang, Zhongyi, Che, Gen, Liu, Liu, Ying, Ao, Lv, Hanli, Han, Lijie, Li, Min, Zhao, Jianyu, Xu, Junqiang, Yang, Zhengan, Zhou, Zhaoyang, Zhang, Xiaolan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845374/
https://www.ncbi.nlm.nih.gov/pubmed/36650145
http://dx.doi.org/10.1038/s41467-023-35936-z
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author Cheng, Zhihua
Liu, Xiaofeng
Yan, Shuangshuang
Liu, Bin
Zhong, Yanting
Song, Weiyuan
Chen, Jiacai
Wang, Zhongyi
Che, Gen
Liu, Liu
Ying, Ao
Lv, Hanli
Han, Lijie
Li, Min
Zhao, Jianyu
Xu, Junqiang
Yang, Zhengan
Zhou, Zhaoyang
Zhang, Xiaolan
author_facet Cheng, Zhihua
Liu, Xiaofeng
Yan, Shuangshuang
Liu, Bin
Zhong, Yanting
Song, Weiyuan
Chen, Jiacai
Wang, Zhongyi
Che, Gen
Liu, Liu
Ying, Ao
Lv, Hanli
Han, Lijie
Li, Min
Zhao, Jianyu
Xu, Junqiang
Yang, Zhengan
Zhou, Zhaoyang
Zhang, Xiaolan
author_sort Cheng, Zhihua
collection PubMed
description Pollen tube guidance within female tissues of flowering plants can be divided into preovular guidance, ovular guidance and a connecting stage called pollen tube emergence. As yet, no female factor has been identified to positively regulate this transition process. In this study, we show that an ovary-expressed bHLH transcription factor Cucumis sativus ALCATRAZ (CsALC) functions in pollen tube emergence in cucumber. CsALC knockout mutants showed diminished pollen tube emergence, extremely reduced entry into ovules, and a 95% reduction in female fertility. Further examination showed two rapid alkalinization factors CsRALF4 and CsRALF19 were less expressed in Csalc ovaries compared to WT. Besides the loss of male fertility derived from precocious pollen tube rupture as in Arabidopsis, Csralf4 Csralf19 double mutants exhibited a 60% decrease in female fertility due to reduced pollen tube distribution and decreased ovule targeting efficiency. In brief, CsALC regulates female fertility and promotes CsRALF4/19 expression in the ovary during pollen tube guidance in cucumber.
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spelling pubmed-98453742023-01-19 Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber Cheng, Zhihua Liu, Xiaofeng Yan, Shuangshuang Liu, Bin Zhong, Yanting Song, Weiyuan Chen, Jiacai Wang, Zhongyi Che, Gen Liu, Liu Ying, Ao Lv, Hanli Han, Lijie Li, Min Zhao, Jianyu Xu, Junqiang Yang, Zhengan Zhou, Zhaoyang Zhang, Xiaolan Nat Commun Article Pollen tube guidance within female tissues of flowering plants can be divided into preovular guidance, ovular guidance and a connecting stage called pollen tube emergence. As yet, no female factor has been identified to positively regulate this transition process. In this study, we show that an ovary-expressed bHLH transcription factor Cucumis sativus ALCATRAZ (CsALC) functions in pollen tube emergence in cucumber. CsALC knockout mutants showed diminished pollen tube emergence, extremely reduced entry into ovules, and a 95% reduction in female fertility. Further examination showed two rapid alkalinization factors CsRALF4 and CsRALF19 were less expressed in Csalc ovaries compared to WT. Besides the loss of male fertility derived from precocious pollen tube rupture as in Arabidopsis, Csralf4 Csralf19 double mutants exhibited a 60% decrease in female fertility due to reduced pollen tube distribution and decreased ovule targeting efficiency. In brief, CsALC regulates female fertility and promotes CsRALF4/19 expression in the ovary during pollen tube guidance in cucumber. Nature Publishing Group UK 2023-01-17 /pmc/articles/PMC9845374/ /pubmed/36650145 http://dx.doi.org/10.1038/s41467-023-35936-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cheng, Zhihua
Liu, Xiaofeng
Yan, Shuangshuang
Liu, Bin
Zhong, Yanting
Song, Weiyuan
Chen, Jiacai
Wang, Zhongyi
Che, Gen
Liu, Liu
Ying, Ao
Lv, Hanli
Han, Lijie
Li, Min
Zhao, Jianyu
Xu, Junqiang
Yang, Zhengan
Zhou, Zhaoyang
Zhang, Xiaolan
Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title_full Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title_fullStr Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title_full_unstemmed Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title_short Pollen tube emergence is mediated by ovary-expressed ALCATRAZ in cucumber
title_sort pollen tube emergence is mediated by ovary-expressed alcatraz in cucumber
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845374/
https://www.ncbi.nlm.nih.gov/pubmed/36650145
http://dx.doi.org/10.1038/s41467-023-35936-z
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