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The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV i...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845923/ https://www.ncbi.nlm.nih.gov/pubmed/36685491 http://dx.doi.org/10.3389/fimmu.2022.1020822 |
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author | Zhang, Yu Xie, Zhiman Zhou, Jie Li, Yanjun Ning, Chuanyi Su, Qisi Ye, Li Ai, Sufang Lai, Jingzhen Pan, Peijiang Liu, Ningmei Liao, Yanyan Su, Qijian Li, Zhuoxin Liang, Hao Cui, Ping Huang, Jiegang |
author_facet | Zhang, Yu Xie, Zhiman Zhou, Jie Li, Yanjun Ning, Chuanyi Su, Qisi Ye, Li Ai, Sufang Lai, Jingzhen Pan, Peijiang Liu, Ningmei Liao, Yanyan Su, Qijian Li, Zhuoxin Liang, Hao Cui, Ping Huang, Jiegang |
author_sort | Zhang, Yu |
collection | PubMed |
description | BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV is seldom reported. METHODS: We conducted a case-controlled study including 41 AIDS patients, 39 pre-AIDS patients and 34 healthy controls. Both AIDS group and pre-AIDS group were divided according to clinical manifestations and CD4 + T cell count. We collected stool samples for 16S rDNA sequencing and untargeted metabolomics analysis, and examined immune activation and microbial translocation for blood samples. RESULTS: The pre-AIDS and AIDS groups had higher levels of microbial translocation and immune activation. There were significant differences in gut microbiota and metabolites at different stages of HIV infection. Higher abundances of pathogenic bacteria or opportunistic pathogen, as well as lower abundances of butyrate-producing bacteria and bacteria with anti-inflammatory potential were associated with HIV severity. The metabolism of tryptophan was disordered after HIV infection. Lower level of anti-inflammatory metabolites and phosphonoacetate, and higher level of phenylethylamine and polyamines were observed in HIV infection. And microbial metabolic pathways related to altered metabolites differed. Moreover, disrupted metabolites contributed by altered microbiota were found to be correlated to microbial translocation and immune activation. CONCLUSIONS: Metabolites caused by dysbiosis of gut microbiota and related metabolic function are correlated to immune activation and microbial translocation, suggesting that the effect of microbiota on metabolites is related to intestinal barrier disruption in HIV infection. |
format | Online Article Text |
id | pubmed-9845923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98459232023-01-19 The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection Zhang, Yu Xie, Zhiman Zhou, Jie Li, Yanjun Ning, Chuanyi Su, Qisi Ye, Li Ai, Sufang Lai, Jingzhen Pan, Peijiang Liu, Ningmei Liao, Yanyan Su, Qijian Li, Zhuoxin Liang, Hao Cui, Ping Huang, Jiegang Front Immunol Immunology BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV is seldom reported. METHODS: We conducted a case-controlled study including 41 AIDS patients, 39 pre-AIDS patients and 34 healthy controls. Both AIDS group and pre-AIDS group were divided according to clinical manifestations and CD4 + T cell count. We collected stool samples for 16S rDNA sequencing and untargeted metabolomics analysis, and examined immune activation and microbial translocation for blood samples. RESULTS: The pre-AIDS and AIDS groups had higher levels of microbial translocation and immune activation. There were significant differences in gut microbiota and metabolites at different stages of HIV infection. Higher abundances of pathogenic bacteria or opportunistic pathogen, as well as lower abundances of butyrate-producing bacteria and bacteria with anti-inflammatory potential were associated with HIV severity. The metabolism of tryptophan was disordered after HIV infection. Lower level of anti-inflammatory metabolites and phosphonoacetate, and higher level of phenylethylamine and polyamines were observed in HIV infection. And microbial metabolic pathways related to altered metabolites differed. Moreover, disrupted metabolites contributed by altered microbiota were found to be correlated to microbial translocation and immune activation. CONCLUSIONS: Metabolites caused by dysbiosis of gut microbiota and related metabolic function are correlated to immune activation and microbial translocation, suggesting that the effect of microbiota on metabolites is related to intestinal barrier disruption in HIV infection. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9845923/ /pubmed/36685491 http://dx.doi.org/10.3389/fimmu.2022.1020822 Text en Copyright © 2023 Zhang, Xie, Zhou, Li, Ning, Su, Ye, Ai, Lai, Pan, Liu, Liao, Su, Li, Liang, Cui and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Zhang, Yu Xie, Zhiman Zhou, Jie Li, Yanjun Ning, Chuanyi Su, Qisi Ye, Li Ai, Sufang Lai, Jingzhen Pan, Peijiang Liu, Ningmei Liao, Yanyan Su, Qijian Li, Zhuoxin Liang, Hao Cui, Ping Huang, Jiegang The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title | The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title_full | The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title_fullStr | The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title_full_unstemmed | The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title_short | The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection |
title_sort | altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during hiv infection |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845923/ https://www.ncbi.nlm.nih.gov/pubmed/36685491 http://dx.doi.org/10.3389/fimmu.2022.1020822 |
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