Cargando…

The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection

BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV i...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yu, Xie, Zhiman, Zhou, Jie, Li, Yanjun, Ning, Chuanyi, Su, Qisi, Ye, Li, Ai, Sufang, Lai, Jingzhen, Pan, Peijiang, Liu, Ningmei, Liao, Yanyan, Su, Qijian, Li, Zhuoxin, Liang, Hao, Cui, Ping, Huang, Jiegang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845923/
https://www.ncbi.nlm.nih.gov/pubmed/36685491
http://dx.doi.org/10.3389/fimmu.2022.1020822
_version_ 1784871029907128320
author Zhang, Yu
Xie, Zhiman
Zhou, Jie
Li, Yanjun
Ning, Chuanyi
Su, Qisi
Ye, Li
Ai, Sufang
Lai, Jingzhen
Pan, Peijiang
Liu, Ningmei
Liao, Yanyan
Su, Qijian
Li, Zhuoxin
Liang, Hao
Cui, Ping
Huang, Jiegang
author_facet Zhang, Yu
Xie, Zhiman
Zhou, Jie
Li, Yanjun
Ning, Chuanyi
Su, Qisi
Ye, Li
Ai, Sufang
Lai, Jingzhen
Pan, Peijiang
Liu, Ningmei
Liao, Yanyan
Su, Qijian
Li, Zhuoxin
Liang, Hao
Cui, Ping
Huang, Jiegang
author_sort Zhang, Yu
collection PubMed
description BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV is seldom reported. METHODS: We conducted a case-controlled study including 41 AIDS patients, 39 pre-AIDS patients and 34 healthy controls. Both AIDS group and pre-AIDS group were divided according to clinical manifestations and CD4 + T cell count. We collected stool samples for 16S rDNA sequencing and untargeted metabolomics analysis, and examined immune activation and microbial translocation for blood samples. RESULTS: The pre-AIDS and AIDS groups had higher levels of microbial translocation and immune activation. There were significant differences in gut microbiota and metabolites at different stages of HIV infection. Higher abundances of pathogenic bacteria or opportunistic pathogen, as well as lower abundances of butyrate-producing bacteria and bacteria with anti-inflammatory potential were associated with HIV severity. The metabolism of tryptophan was disordered after HIV infection. Lower level of anti-inflammatory metabolites and phosphonoacetate, and higher level of phenylethylamine and polyamines were observed in HIV infection. And microbial metabolic pathways related to altered metabolites differed. Moreover, disrupted metabolites contributed by altered microbiota were found to be correlated to microbial translocation and immune activation. CONCLUSIONS: Metabolites caused by dysbiosis of gut microbiota and related metabolic function are correlated to immune activation and microbial translocation, suggesting that the effect of microbiota on metabolites is related to intestinal barrier disruption in HIV infection.
format Online
Article
Text
id pubmed-9845923
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98459232023-01-19 The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection Zhang, Yu Xie, Zhiman Zhou, Jie Li, Yanjun Ning, Chuanyi Su, Qisi Ye, Li Ai, Sufang Lai, Jingzhen Pan, Peijiang Liu, Ningmei Liao, Yanyan Su, Qijian Li, Zhuoxin Liang, Hao Cui, Ping Huang, Jiegang Front Immunol Immunology BACKGROUND: The immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV is seldom reported. METHODS: We conducted a case-controlled study including 41 AIDS patients, 39 pre-AIDS patients and 34 healthy controls. Both AIDS group and pre-AIDS group were divided according to clinical manifestations and CD4 + T cell count. We collected stool samples for 16S rDNA sequencing and untargeted metabolomics analysis, and examined immune activation and microbial translocation for blood samples. RESULTS: The pre-AIDS and AIDS groups had higher levels of microbial translocation and immune activation. There were significant differences in gut microbiota and metabolites at different stages of HIV infection. Higher abundances of pathogenic bacteria or opportunistic pathogen, as well as lower abundances of butyrate-producing bacteria and bacteria with anti-inflammatory potential were associated with HIV severity. The metabolism of tryptophan was disordered after HIV infection. Lower level of anti-inflammatory metabolites and phosphonoacetate, and higher level of phenylethylamine and polyamines were observed in HIV infection. And microbial metabolic pathways related to altered metabolites differed. Moreover, disrupted metabolites contributed by altered microbiota were found to be correlated to microbial translocation and immune activation. CONCLUSIONS: Metabolites caused by dysbiosis of gut microbiota and related metabolic function are correlated to immune activation and microbial translocation, suggesting that the effect of microbiota on metabolites is related to intestinal barrier disruption in HIV infection. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9845923/ /pubmed/36685491 http://dx.doi.org/10.3389/fimmu.2022.1020822 Text en Copyright © 2023 Zhang, Xie, Zhou, Li, Ning, Su, Ye, Ai, Lai, Pan, Liu, Liao, Su, Li, Liang, Cui and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhang, Yu
Xie, Zhiman
Zhou, Jie
Li, Yanjun
Ning, Chuanyi
Su, Qisi
Ye, Li
Ai, Sufang
Lai, Jingzhen
Pan, Peijiang
Liu, Ningmei
Liao, Yanyan
Su, Qijian
Li, Zhuoxin
Liang, Hao
Cui, Ping
Huang, Jiegang
The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title_full The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title_fullStr The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title_full_unstemmed The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title_short The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection
title_sort altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during hiv infection
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845923/
https://www.ncbi.nlm.nih.gov/pubmed/36685491
http://dx.doi.org/10.3389/fimmu.2022.1020822
work_keys_str_mv AT zhangyu thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT xiezhiman thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT zhoujie thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liyanjun thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT ningchuanyi thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT suqisi thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT yeli thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT aisufang thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT laijingzhen thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT panpeijiang thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liuningmei thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liaoyanyan thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT suqijian thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT lizhuoxin thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT lianghao thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT cuiping thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT huangjiegang thealteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT zhangyu alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT xiezhiman alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT zhoujie alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liyanjun alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT ningchuanyi alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT suqisi alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT yeli alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT aisufang alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT laijingzhen alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT panpeijiang alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liuningmei alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT liaoyanyan alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT suqijian alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT lizhuoxin alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT lianghao alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT cuiping alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection
AT huangjiegang alteredmetabolitescontributedbydysbiosisofgutmicrobiotaareassociatedwithmicrobialtranslocationandimmuneactivationduringhivinfection