Protocol for standardized intrathymic injection in mice

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injecti...

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Detalles Bibliográficos
Autores principales: Tsai, Tsung-Lin, Tsai, Pei-Yuan, Dzhagalov, Ivan L., Hsu, Chia-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845999/
https://www.ncbi.nlm.nih.gov/pubmed/36638012
http://dx.doi.org/10.1016/j.xpro.2022.102010
Descripción
Sumario:Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).(1)

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