Protocol for standardized intrathymic injection in mice

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injecti...

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Detalles Bibliográficos
Autores principales: Tsai, Tsung-Lin, Tsai, Pei-Yuan, Dzhagalov, Ivan L., Hsu, Chia-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845999/
https://www.ncbi.nlm.nih.gov/pubmed/36638012
http://dx.doi.org/10.1016/j.xpro.2022.102010
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author Tsai, Tsung-Lin
Tsai, Pei-Yuan
Dzhagalov, Ivan L.
Hsu, Chia-Lin
author_facet Tsai, Tsung-Lin
Tsai, Pei-Yuan
Dzhagalov, Ivan L.
Hsu, Chia-Lin
author_sort Tsai, Tsung-Lin
collection PubMed
description Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).(1)
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spelling pubmed-98459992023-01-19 Protocol for standardized intrathymic injection in mice Tsai, Tsung-Lin Tsai, Pei-Yuan Dzhagalov, Ivan L. Hsu, Chia-Lin STAR Protoc Protocol Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).(1) Elsevier 2023-01-12 /pmc/articles/PMC9845999/ /pubmed/36638012 http://dx.doi.org/10.1016/j.xpro.2022.102010 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tsai, Tsung-Lin
Tsai, Pei-Yuan
Dzhagalov, Ivan L.
Hsu, Chia-Lin
Protocol for standardized intrathymic injection in mice
title Protocol for standardized intrathymic injection in mice
title_full Protocol for standardized intrathymic injection in mice
title_fullStr Protocol for standardized intrathymic injection in mice
title_full_unstemmed Protocol for standardized intrathymic injection in mice
title_short Protocol for standardized intrathymic injection in mice
title_sort protocol for standardized intrathymic injection in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845999/
https://www.ncbi.nlm.nih.gov/pubmed/36638012
http://dx.doi.org/10.1016/j.xpro.2022.102010
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