Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos

Taming gene expression variability is critical for robust pattern formation during embryonic development. Here, we describe an optimized protocol for single-molecule fluorescence in situ hybridization and immunohistochemistry in zebrafish embryos. We detail how to count segmentation clock RNAs and c...

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Detalles Bibliográficos
Autores principales: Keseroglu, Kemal, Zinani, Oriana Q.H., Özbudak, Ertuğrul M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846013/
https://www.ncbi.nlm.nih.gov/pubmed/36638016
http://dx.doi.org/10.1016/j.xpro.2022.102020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846013/
https://www.ncbi.nlm.nih.gov/pubmed/36638016
http://dx.doi.org/10.1016/j.xpro.2022.102020

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