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Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture

The inhibitory effect of growth hormone (GH) on adipose tissue growth and the stimulatory effect of GH on lipolysis are well known, but the mechanisms underlying these effects are not completely understood. In this study, we revisited the effects of GH on adipose tissue growth and lipolysis in the l...

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Autores principales: Zhao, Lidan, Jiang, Honglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846043/
https://www.ncbi.nlm.nih.gov/pubmed/36686475
http://dx.doi.org/10.3389/fendo.2022.1028191
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author Zhao, Lidan
Jiang, Honglin
author_facet Zhao, Lidan
Jiang, Honglin
author_sort Zhao, Lidan
collection PubMed
description The inhibitory effect of growth hormone (GH) on adipose tissue growth and the stimulatory effect of GH on lipolysis are well known, but the mechanisms underlying these effects are not completely understood. In this study, we revisited the effects of GH on adipose tissue growth and lipolysis in the lit/lit mouse model. The lit/lit mice are GH deficient because of a mutation in the GH releasing hormone receptor gene. We found that the lit/lit mice had more subcutaneous fat and larger adipocytes than their heterozygous lit/+ littermates and that these differences were partially reversed by 4-week GH injection. We also found that GH injection to the lit/lit mice caused the mature adipose tissue and adipocytes to reduce in size. These results demonstrate that GH inhibits adipose tissue growth at least in part by stimulating lipolysis. To determine the mechanism by which GH stimulates lipolysis, we cultured adipose tissue explants and adipocytes derived from lit/lit mice with GH and/or isoproterenol, an agonist of the beta-adrenergic receptors. These experiments showed that whereas isoproterenol, expectedly, stimulated potent lipolysis, GH, surprisingly, had no effect on basal lipolysis or isoproterenol-induced lipolysis in adipose tissue explants or adipocytes. We also found that both isoproterenol-induced lipolysis and phosphorylation of hormone-sensitive lipase were not different between lit/lit and lit/+ mice. Taken together, these results support the conclusion that GH has lipolytic effect in mice but argue against the notion that GH stimulates lipolysis by directly acting on adipocytes or by enhancing β-adrenergic receptors-mediated lipolysis.
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spelling pubmed-98460432023-01-19 Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture Zhao, Lidan Jiang, Honglin Front Endocrinol (Lausanne) Endocrinology The inhibitory effect of growth hormone (GH) on adipose tissue growth and the stimulatory effect of GH on lipolysis are well known, but the mechanisms underlying these effects are not completely understood. In this study, we revisited the effects of GH on adipose tissue growth and lipolysis in the lit/lit mouse model. The lit/lit mice are GH deficient because of a mutation in the GH releasing hormone receptor gene. We found that the lit/lit mice had more subcutaneous fat and larger adipocytes than their heterozygous lit/+ littermates and that these differences were partially reversed by 4-week GH injection. We also found that GH injection to the lit/lit mice caused the mature adipose tissue and adipocytes to reduce in size. These results demonstrate that GH inhibits adipose tissue growth at least in part by stimulating lipolysis. To determine the mechanism by which GH stimulates lipolysis, we cultured adipose tissue explants and adipocytes derived from lit/lit mice with GH and/or isoproterenol, an agonist of the beta-adrenergic receptors. These experiments showed that whereas isoproterenol, expectedly, stimulated potent lipolysis, GH, surprisingly, had no effect on basal lipolysis or isoproterenol-induced lipolysis in adipose tissue explants or adipocytes. We also found that both isoproterenol-induced lipolysis and phosphorylation of hormone-sensitive lipase were not different between lit/lit and lit/+ mice. Taken together, these results support the conclusion that GH has lipolytic effect in mice but argue against the notion that GH stimulates lipolysis by directly acting on adipocytes or by enhancing β-adrenergic receptors-mediated lipolysis. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9846043/ /pubmed/36686475 http://dx.doi.org/10.3389/fendo.2022.1028191 Text en Copyright © 2023 Zhao and Jiang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Zhao, Lidan
Jiang, Honglin
Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title_full Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title_fullStr Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title_full_unstemmed Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title_short Growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
title_sort growth hormone stimulates lipolysis in mice but not in adipose tissue or adipocyte culture
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846043/
https://www.ncbi.nlm.nih.gov/pubmed/36686475
http://dx.doi.org/10.3389/fendo.2022.1028191
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