Cargando…
The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis
OBJECTIVE: Helicobacter pylori is planted in the human stomach and is the most common cause of chronic gastritis, which produced specific local and systemic humoral immunity, while the associations of these immune responses and H. pylori in the development of chronic gastritis remain unclear. METHOD...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846114/ https://www.ncbi.nlm.nih.gov/pubmed/36705412 http://dx.doi.org/10.1002/iid3.768 |
_version_ | 1784871085258309632 |
---|---|
author | Yao, Biyu Xu, Xiaoyan Liu, Weijie Zhang, Qin Wang, Wei Huang, Zhiming |
author_facet | Yao, Biyu Xu, Xiaoyan Liu, Weijie Zhang, Qin Wang, Wei Huang, Zhiming |
author_sort | Yao, Biyu |
collection | PubMed |
description | OBJECTIVE: Helicobacter pylori is planted in the human stomach and is the most common cause of chronic gastritis, which produced specific local and systemic humoral immunity, while the associations of these immune responses and H. pylori in the development of chronic gastritis remain unclear. METHODS: This study analyzed histology, the number of Th22 and regulatory T (Treg) cells, and the levels of inflammation‐ and gastritis‐related indicators between 22 H. pylori‐infected and 24 non‐H. pylori‐infected chronic gastritis patients by hematoxylin‐eosin staining, enzyme‐linked immunosorbent assay, quantitative reverse transcription PCR, and flow cytometry analysis. RESULTS: This study found that the pathological damage degree of gastric mucosa in H. pylori infection patients was more serious. In the H. pylori‐infected patient serum, the gastrin, G‐17, interleukins (IL)‐22, transforming growth factor (TGF)‐β, tumor necrosis factor (TNF)‐α, IL‐4, and IL‐17A levels were notably raised, while the interferon (IFN)‐γ level was inhibited, and in gastric mucosa, and except IFN‐γ, the IL‐22, forkhead box P3 (Foxp3), TNF‐α, IL‐4, and IL‐17A mRNA levels were raised too. The receiver operating characteristic curve analysis indicates serum IL‐22, TGF‐β, TNF‐α, IL‐4, and IL‐17A are suitable for differential diagnosis of H. pylori infection. In addition, in the peripheral blood, the percentages of the IL‐22(+) CD4(+) and Foxp3(+) CD4(+) T cells were raised with H. pylori infection. The positive correlation between IL‐22 and Foxp3 mRNA levels and the degree of H. pylori colonization and gastric mucositis by Pearson's correlation analysis. CONCLUSIONS: Treg and Th22 cells were positively associated with the degree of H. pylori infection and the severity of gastritis. In summary, this study provides an experimental basis for the study of the eradication of H. pylori and the biological mechanism of chronic gastritis. |
format | Online Article Text |
id | pubmed-9846114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98461142023-01-24 The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis Yao, Biyu Xu, Xiaoyan Liu, Weijie Zhang, Qin Wang, Wei Huang, Zhiming Immun Inflamm Dis Original Articles OBJECTIVE: Helicobacter pylori is planted in the human stomach and is the most common cause of chronic gastritis, which produced specific local and systemic humoral immunity, while the associations of these immune responses and H. pylori in the development of chronic gastritis remain unclear. METHODS: This study analyzed histology, the number of Th22 and regulatory T (Treg) cells, and the levels of inflammation‐ and gastritis‐related indicators between 22 H. pylori‐infected and 24 non‐H. pylori‐infected chronic gastritis patients by hematoxylin‐eosin staining, enzyme‐linked immunosorbent assay, quantitative reverse transcription PCR, and flow cytometry analysis. RESULTS: This study found that the pathological damage degree of gastric mucosa in H. pylori infection patients was more serious. In the H. pylori‐infected patient serum, the gastrin, G‐17, interleukins (IL)‐22, transforming growth factor (TGF)‐β, tumor necrosis factor (TNF)‐α, IL‐4, and IL‐17A levels were notably raised, while the interferon (IFN)‐γ level was inhibited, and in gastric mucosa, and except IFN‐γ, the IL‐22, forkhead box P3 (Foxp3), TNF‐α, IL‐4, and IL‐17A mRNA levels were raised too. The receiver operating characteristic curve analysis indicates serum IL‐22, TGF‐β, TNF‐α, IL‐4, and IL‐17A are suitable for differential diagnosis of H. pylori infection. In addition, in the peripheral blood, the percentages of the IL‐22(+) CD4(+) and Foxp3(+) CD4(+) T cells were raised with H. pylori infection. The positive correlation between IL‐22 and Foxp3 mRNA levels and the degree of H. pylori colonization and gastric mucositis by Pearson's correlation analysis. CONCLUSIONS: Treg and Th22 cells were positively associated with the degree of H. pylori infection and the severity of gastritis. In summary, this study provides an experimental basis for the study of the eradication of H. pylori and the biological mechanism of chronic gastritis. John Wiley and Sons Inc. 2023-01-18 /pmc/articles/PMC9846114/ /pubmed/36705412 http://dx.doi.org/10.1002/iid3.768 Text en © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Yao, Biyu Xu, Xiaoyan Liu, Weijie Zhang, Qin Wang, Wei Huang, Zhiming The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title | The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title_full | The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title_fullStr | The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title_full_unstemmed | The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title_short | The correlation of Th22 and regulatory T cells with Helicobacter pylori infection in patients with chronic gastritis |
title_sort | correlation of th22 and regulatory t cells with helicobacter pylori infection in patients with chronic gastritis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846114/ https://www.ncbi.nlm.nih.gov/pubmed/36705412 http://dx.doi.org/10.1002/iid3.768 |
work_keys_str_mv | AT yaobiyu thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT xuxiaoyan thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT liuweijie thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT zhangqin thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT wangwei thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT huangzhiming thecorrelationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT yaobiyu correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT xuxiaoyan correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT liuweijie correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT zhangqin correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT wangwei correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis AT huangzhiming correlationofth22andregulatorytcellswithhelicobacterpyloriinfectioninpatientswithchronicgastritis |