Protocol to establish a mouse model for hepatic metastasis of colorectal cancer

Hepatic metastasis is the leading cause of colorectal-cancer (CRC)-associated death. Here we describe an optimized protocol to establish a more clinically relevant mouse model of CRC metastasis. We detail steps for subcutaneous transplantation of luciferase-expressing CRC cells and subsequent orthot...

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Detalles Bibliográficos
Autores principales: Zhang, Yajuan, Zhao, Ming, Yang, Weiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846120/
https://www.ncbi.nlm.nih.gov/pubmed/36640367
http://dx.doi.org/10.1016/j.xpro.2022.102023
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author Zhang, Yajuan
Zhao, Ming
Yang, Weiwei
author_facet Zhang, Yajuan
Zhao, Ming
Yang, Weiwei
author_sort Zhang, Yajuan
collection PubMed
description Hepatic metastasis is the leading cause of colorectal-cancer (CRC)-associated death. Here we describe an optimized protocol to establish a more clinically relevant mouse model of CRC metastasis. We detail steps for subcutaneous transplantation of luciferase-expressing CRC cells and subsequent orthotopic transplantation of subcutaneous tumor tissues. This mouse model allows CRC cells to form tumors within the intestinal tract and metastasize to the liver, thereby providing the approach to assess hepatic metastasis of CRC in vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).(1)
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spelling pubmed-98461202023-01-19 Protocol to establish a mouse model for hepatic metastasis of colorectal cancer Zhang, Yajuan Zhao, Ming Yang, Weiwei STAR Protoc Protocol Hepatic metastasis is the leading cause of colorectal-cancer (CRC)-associated death. Here we describe an optimized protocol to establish a more clinically relevant mouse model of CRC metastasis. We detail steps for subcutaneous transplantation of luciferase-expressing CRC cells and subsequent orthotopic transplantation of subcutaneous tumor tissues. This mouse model allows CRC cells to form tumors within the intestinal tract and metastasize to the liver, thereby providing the approach to assess hepatic metastasis of CRC in vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).(1) Elsevier 2023-01-12 /pmc/articles/PMC9846120/ /pubmed/36640367 http://dx.doi.org/10.1016/j.xpro.2022.102023 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Yajuan
Zhao, Ming
Yang, Weiwei
Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title_full Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title_fullStr Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title_full_unstemmed Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title_short Protocol to establish a mouse model for hepatic metastasis of colorectal cancer
title_sort protocol to establish a mouse model for hepatic metastasis of colorectal cancer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846120/
https://www.ncbi.nlm.nih.gov/pubmed/36640367
http://dx.doi.org/10.1016/j.xpro.2022.102023
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