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Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application
Neurotrophin-4 (NT-4) is a neurotrophic factor that plays important roles in maintaining nerve cell survival, regulating neuronal differentiation and apoptosis, and promoting nerve injury repair. However, the source of sufficient NT-4 protein and efficient delivery of NT-4 remain a challenge. This s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846172/ https://www.ncbi.nlm.nih.gov/pubmed/36685209 http://dx.doi.org/10.3389/fphys.2022.1104929 |
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author | Zhang, Wenchang Li, Zhiqing Lan, Weiqun Guo, Hao Chen, Feng Wang, Feng Shen, Guanwang Xia, Qingyou Zhao, Ping |
author_facet | Zhang, Wenchang Li, Zhiqing Lan, Weiqun Guo, Hao Chen, Feng Wang, Feng Shen, Guanwang Xia, Qingyou Zhao, Ping |
author_sort | Zhang, Wenchang |
collection | PubMed |
description | Neurotrophin-4 (NT-4) is a neurotrophic factor that plays important roles in maintaining nerve cell survival, regulating neuronal differentiation and apoptosis, and promoting nerve injury repair. However, the source of sufficient NT-4 protein and efficient delivery of NT-4 remain a challenge. This study aims to express an activated human NT-4 protein in a large scale by genetically engineering silk gland bioreactor of silkworm as a host. We showed that the expression of human NT-4-functionalized silk material could promote proliferation of mouse HT22 cells when compared to the natural silk protein, and no obvious cytotoxicity was observed under the conditions of different silk materials. Importantly, this functional silk material was able to induce the potential differentiation of HT22 cells, promote peripheral neural cell migration and neurite outgrowth of chicken embryo dorsal root ganglion (DRG). All these results demonstrated a high bioactivity of human NT-4 protein produced in silk gland. Therefore, based on the silkworm model, the further fabrication of different silk materials-carrying active NT-4 protein with good mechanical properties and great biocompatibility will give promising applications in tissue engineering and neurons regeneration. |
format | Online Article Text |
id | pubmed-9846172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98461722023-01-19 Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application Zhang, Wenchang Li, Zhiqing Lan, Weiqun Guo, Hao Chen, Feng Wang, Feng Shen, Guanwang Xia, Qingyou Zhao, Ping Front Physiol Physiology Neurotrophin-4 (NT-4) is a neurotrophic factor that plays important roles in maintaining nerve cell survival, regulating neuronal differentiation and apoptosis, and promoting nerve injury repair. However, the source of sufficient NT-4 protein and efficient delivery of NT-4 remain a challenge. This study aims to express an activated human NT-4 protein in a large scale by genetically engineering silk gland bioreactor of silkworm as a host. We showed that the expression of human NT-4-functionalized silk material could promote proliferation of mouse HT22 cells when compared to the natural silk protein, and no obvious cytotoxicity was observed under the conditions of different silk materials. Importantly, this functional silk material was able to induce the potential differentiation of HT22 cells, promote peripheral neural cell migration and neurite outgrowth of chicken embryo dorsal root ganglion (DRG). All these results demonstrated a high bioactivity of human NT-4 protein produced in silk gland. Therefore, based on the silkworm model, the further fabrication of different silk materials-carrying active NT-4 protein with good mechanical properties and great biocompatibility will give promising applications in tissue engineering and neurons regeneration. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9846172/ /pubmed/36685209 http://dx.doi.org/10.3389/fphys.2022.1104929 Text en Copyright © 2023 Zhang, Li, Lan, Guo, Chen, Wang, Shen, Xia and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Zhang, Wenchang Li, Zhiqing Lan, Weiqun Guo, Hao Chen, Feng Wang, Feng Shen, Guanwang Xia, Qingyou Zhao, Ping Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title | Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title_full | Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title_fullStr | Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title_full_unstemmed | Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title_short | Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
title_sort | bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846172/ https://www.ncbi.nlm.nih.gov/pubmed/36685209 http://dx.doi.org/10.3389/fphys.2022.1104929 |
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