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Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China

The double selection of environment adaptation and host specificity forced the diversification of rhizobia in nature. In the tropical region of China, Medicago polymorpha and Medicago lupulina are widely distributed, particularly in purple soil. However, the local distribution and diversity of rhizo...

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Autores principales: Tang, Mingxing, Wang, Haoyu, Qi, Xin, He, Teng, Zhang, Bin, Wang, Entao, Yu, Miao, Wang, Beinan, Wang, Fang, Liu, Zhongkuan, Liu, Xiaoyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846747/
https://www.ncbi.nlm.nih.gov/pubmed/36687603
http://dx.doi.org/10.3389/fmicb.2022.1055694
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author Tang, Mingxing
Wang, Haoyu
Qi, Xin
He, Teng
Zhang, Bin
Wang, Entao
Yu, Miao
Wang, Beinan
Wang, Fang
Liu, Zhongkuan
Liu, Xiaoyun
author_facet Tang, Mingxing
Wang, Haoyu
Qi, Xin
He, Teng
Zhang, Bin
Wang, Entao
Yu, Miao
Wang, Beinan
Wang, Fang
Liu, Zhongkuan
Liu, Xiaoyun
author_sort Tang, Mingxing
collection PubMed
description The double selection of environment adaptation and host specificity forced the diversification of rhizobia in nature. In the tropical region of China, Medicago polymorpha and Medicago lupulina are widely distributed, particularly in purple soil. However, the local distribution and diversity of rhizobia associated with these legumes has not been systematically investigated. To this end, root nodules of M. polymorpha and M. lupulina grown in purple soil at seven locations in Yunnan Province of China were collected for rhizobial isolation. The obtained rhizobia were characterized by RFLP of 16S–23S rRNA intergenic spacer, BOXAIR fingerprinting, and phylogeny of housekeeping and symbiosis genes. As result, a total of 91 rhizobial strains were classified into species Sinorhizobium medicae and S. meliloti, while three nodC gene types were identified among them. S. medicae containing nodC of type I was dominant in farmlands associated with M. polymorpha; while S. meliloti harboring nodC of type III was dominant in wild land nodulated by M. lupulina. For both rhizobial species, greater genetic diversity was detected in the populations isolated from their preferred host plant. A high level of genetic differentiation was observed between the two Sinorhizobium species, and gene flow was evident within the populations of the same species derived from different soil types, indicating that rhizobial evolution is likely associated with the soil features. To examine the effects of environmental features on rhizobial distribution, soil physicochemical traits and rhizobial genotypes were applied for constrained analysis of principle coordinates, which demonstrated that soil features like pH, nitrogen and sodium were the principle factors governing the rhizobial geographical distribution. Altogether, both S. medicae and S. meliloti strains could naturally nodulate with M. polymorpha and M. lupulina, but the rhizobium-legume symbiosis compatibility determined by both the host species and soil factors was also highlighted.
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spelling pubmed-98467472023-01-19 Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China Tang, Mingxing Wang, Haoyu Qi, Xin He, Teng Zhang, Bin Wang, Entao Yu, Miao Wang, Beinan Wang, Fang Liu, Zhongkuan Liu, Xiaoyun Front Microbiol Microbiology The double selection of environment adaptation and host specificity forced the diversification of rhizobia in nature. In the tropical region of China, Medicago polymorpha and Medicago lupulina are widely distributed, particularly in purple soil. However, the local distribution and diversity of rhizobia associated with these legumes has not been systematically investigated. To this end, root nodules of M. polymorpha and M. lupulina grown in purple soil at seven locations in Yunnan Province of China were collected for rhizobial isolation. The obtained rhizobia were characterized by RFLP of 16S–23S rRNA intergenic spacer, BOXAIR fingerprinting, and phylogeny of housekeeping and symbiosis genes. As result, a total of 91 rhizobial strains were classified into species Sinorhizobium medicae and S. meliloti, while three nodC gene types were identified among them. S. medicae containing nodC of type I was dominant in farmlands associated with M. polymorpha; while S. meliloti harboring nodC of type III was dominant in wild land nodulated by M. lupulina. For both rhizobial species, greater genetic diversity was detected in the populations isolated from their preferred host plant. A high level of genetic differentiation was observed between the two Sinorhizobium species, and gene flow was evident within the populations of the same species derived from different soil types, indicating that rhizobial evolution is likely associated with the soil features. To examine the effects of environmental features on rhizobial distribution, soil physicochemical traits and rhizobial genotypes were applied for constrained analysis of principle coordinates, which demonstrated that soil features like pH, nitrogen and sodium were the principle factors governing the rhizobial geographical distribution. Altogether, both S. medicae and S. meliloti strains could naturally nodulate with M. polymorpha and M. lupulina, but the rhizobium-legume symbiosis compatibility determined by both the host species and soil factors was also highlighted. Frontiers Media S.A. 2023-01-04 /pmc/articles/PMC9846747/ /pubmed/36687603 http://dx.doi.org/10.3389/fmicb.2022.1055694 Text en Copyright © 2023 Tang, Wang, Qi, He, Zhang, Wang, Yu, Wang, Wang, Liu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tang, Mingxing
Wang, Haoyu
Qi, Xin
He, Teng
Zhang, Bin
Wang, Entao
Yu, Miao
Wang, Beinan
Wang, Fang
Liu, Zhongkuan
Liu, Xiaoyun
Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title_full Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title_fullStr Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title_full_unstemmed Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title_short Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China
title_sort diversification of sinorhizobium populations associated with medicago polymorpha and medicago lupulina in purple soil of china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846747/
https://www.ncbi.nlm.nih.gov/pubmed/36687603
http://dx.doi.org/10.3389/fmicb.2022.1055694
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