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Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study

INTRODUCTION: Studies exploring alterations in blood coagulation and platelet activation induced by COVID-19 vaccines are not concordant. We aimed to assess the impact of four COVID-19 vaccines on platelet activation, coagulation, and inflammation considering also the immunization dose and the histo...

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Autores principales: Brambilla, Marta, Canzano, Paola, Valle, Patrizia Della, Becchetti, Alessia, Conti, Maria, Alberti, Mariangela, Galotta, Arianna, Biondi, Maria Luisa, Lonati, Paola Adele, Veglia, Fabrizio, Bonomi, Alice, Cosentino, Nicola, Meroni, Pier Luigi, Zuccotti, Gian Vincenzo, D'Angelo, Armando, Camera, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846886/
https://www.ncbi.nlm.nih.gov/pubmed/36702064
http://dx.doi.org/10.1016/j.thromres.2023.01.015
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author Brambilla, Marta
Canzano, Paola
Valle, Patrizia Della
Becchetti, Alessia
Conti, Maria
Alberti, Mariangela
Galotta, Arianna
Biondi, Maria Luisa
Lonati, Paola Adele
Veglia, Fabrizio
Bonomi, Alice
Cosentino, Nicola
Meroni, Pier Luigi
Zuccotti, Gian Vincenzo
D'Angelo, Armando
Camera, Marina
author_facet Brambilla, Marta
Canzano, Paola
Valle, Patrizia Della
Becchetti, Alessia
Conti, Maria
Alberti, Mariangela
Galotta, Arianna
Biondi, Maria Luisa
Lonati, Paola Adele
Veglia, Fabrizio
Bonomi, Alice
Cosentino, Nicola
Meroni, Pier Luigi
Zuccotti, Gian Vincenzo
D'Angelo, Armando
Camera, Marina
author_sort Brambilla, Marta
collection PubMed
description INTRODUCTION: Studies exploring alterations in blood coagulation and platelet activation induced by COVID-19 vaccines are not concordant. We aimed to assess the impact of four COVID-19 vaccines on platelet activation, coagulation, and inflammation considering also the immunization dose and the history of SARS-CoV-2 infection. METHODS: TREASURE study enrolled 368 consecutive subjects (161 receiving viral vector vaccines -ChAdOx1-S/Vaxzevria or Janssen- and 207 receiving mRNA vaccines -Comirnaty/Pfizer-BioNTech or Spikevax/Moderna). Blood was collected the day before and 8 ± 2 days after the vaccination. Platelet activation markers (P-selectin, aGPIIbIIIa and Tissue Factor expression; number of platelet-monocyte and -granulocyte aggregates) and microvesicle release were analyzed by flow cytometry. Platelet thrombin generation (TG) capacity was measured using the Calibrated Automated Thrombogram. Plasma coagulation and inflammation markers and immune response were evaluated by ELISA. RESULTS: Vaccination did not induce platelet activation and microvesicle release. IL-6 and CRP levels (+30%), D-dimer, fibrinogen and F(1)(+2) (+13%, +3.7%, +4.3%, respectively) but not TAT levels significantly increased upon immunization with all four vaccines, with no difference among them and between first and second dose. An overall minor post-vaccination reduction of aPC, TM and TFPI, all possibly related to endothelial function, was observed. No anti-PF4 seroconversion was observed. CONCLUSION: This study showed that the four COVID-19 vaccines administered to a large population sample induce a transient inflammatory response, with no onset of platelet activation. The minor changes in clotting activation and endothelial function might be potentially involved at a population level in explaining the very rare venous thromboembolic complications of COVID-19 vaccination.
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spelling pubmed-98468862023-01-18 Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study Brambilla, Marta Canzano, Paola Valle, Patrizia Della Becchetti, Alessia Conti, Maria Alberti, Mariangela Galotta, Arianna Biondi, Maria Luisa Lonati, Paola Adele Veglia, Fabrizio Bonomi, Alice Cosentino, Nicola Meroni, Pier Luigi Zuccotti, Gian Vincenzo D'Angelo, Armando Camera, Marina Thromb Res Full Length Article INTRODUCTION: Studies exploring alterations in blood coagulation and platelet activation induced by COVID-19 vaccines are not concordant. We aimed to assess the impact of four COVID-19 vaccines on platelet activation, coagulation, and inflammation considering also the immunization dose and the history of SARS-CoV-2 infection. METHODS: TREASURE study enrolled 368 consecutive subjects (161 receiving viral vector vaccines -ChAdOx1-S/Vaxzevria or Janssen- and 207 receiving mRNA vaccines -Comirnaty/Pfizer-BioNTech or Spikevax/Moderna). Blood was collected the day before and 8 ± 2 days after the vaccination. Platelet activation markers (P-selectin, aGPIIbIIIa and Tissue Factor expression; number of platelet-monocyte and -granulocyte aggregates) and microvesicle release were analyzed by flow cytometry. Platelet thrombin generation (TG) capacity was measured using the Calibrated Automated Thrombogram. Plasma coagulation and inflammation markers and immune response were evaluated by ELISA. RESULTS: Vaccination did not induce platelet activation and microvesicle release. IL-6 and CRP levels (+30%), D-dimer, fibrinogen and F(1)(+2) (+13%, +3.7%, +4.3%, respectively) but not TAT levels significantly increased upon immunization with all four vaccines, with no difference among them and between first and second dose. An overall minor post-vaccination reduction of aPC, TM and TFPI, all possibly related to endothelial function, was observed. No anti-PF4 seroconversion was observed. CONCLUSION: This study showed that the four COVID-19 vaccines administered to a large population sample induce a transient inflammatory response, with no onset of platelet activation. The minor changes in clotting activation and endothelial function might be potentially involved at a population level in explaining the very rare venous thromboembolic complications of COVID-19 vaccination. The Authors. Published by Elsevier Ltd. 2023-03 2023-01-18 /pmc/articles/PMC9846886/ /pubmed/36702064 http://dx.doi.org/10.1016/j.thromres.2023.01.015 Text en © 2023 The Authors. Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Full Length Article
Brambilla, Marta
Canzano, Paola
Valle, Patrizia Della
Becchetti, Alessia
Conti, Maria
Alberti, Mariangela
Galotta, Arianna
Biondi, Maria Luisa
Lonati, Paola Adele
Veglia, Fabrizio
Bonomi, Alice
Cosentino, Nicola
Meroni, Pier Luigi
Zuccotti, Gian Vincenzo
D'Angelo, Armando
Camera, Marina
Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title_full Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title_fullStr Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title_full_unstemmed Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title_short Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study
title_sort head-to-head comparison of four covid-19 vaccines on platelet activation, coagulation and inflammation. the treasure study
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846886/
https://www.ncbi.nlm.nih.gov/pubmed/36702064
http://dx.doi.org/10.1016/j.thromres.2023.01.015
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