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3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone

Osteochondral defect caused by trauma or osteoarthritis exhibits a major challenge in clinical treatment with limited symptomatic effects at present. The regeneration and remodeling of subchondral bone play a positive effect on cartilage regeneration and further promotes the repair of osteochondral...

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Autores principales: Cao, Yanyan, Sun, Lei, Liu, Zixian, Shen, Zhizhong, Jia, Wendan, Hou, Peiyi, Sang, Shengbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847519/
https://www.ncbi.nlm.nih.gov/pubmed/36683741
http://dx.doi.org/10.1093/rb/rbac104
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author Cao, Yanyan
Sun, Lei
Liu, Zixian
Shen, Zhizhong
Jia, Wendan
Hou, Peiyi
Sang, Shengbo
author_facet Cao, Yanyan
Sun, Lei
Liu, Zixian
Shen, Zhizhong
Jia, Wendan
Hou, Peiyi
Sang, Shengbo
author_sort Cao, Yanyan
collection PubMed
description Osteochondral defect caused by trauma or osteoarthritis exhibits a major challenge in clinical treatment with limited symptomatic effects at present. The regeneration and remodeling of subchondral bone play a positive effect on cartilage regeneration and further promotes the repair of osteochondral defects. Making use of the strengths of each preparation method, the combination of 3D printing and electrospinning is a promising method for designing and constructing multi-scale scaffolds that mimic the complexity and hierarchical structure of subchondral bone at the microscale and nanoscale, respectively. In this study, the 3D printed-electrospun poly(ɛ-caprolactone)/nano-hydroxyapatites/multi-walled carbon nanotubes (PCL/nHA/MWCNTs) scaffolds were successfully constructed by the combination of electrospinning and layer-by-layer 3D printing. The resulting dual-scale scaffold consisted of a dense layer of disordered nanospun fibers and a porous microscale 3D scaffold layer to support and promote the ingrowth of subchondral bone. Herein, the biomimetic PCL/nHA/MWCNTs scaffolds enhanced cell seeding efficiency and allowed for higher cell–cell interactions that supported the adhesion, proliferation, activity, morphology and subsequently improved the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Together, this study elucidates that the construction of 3D printed-electrospun PCL/nHA/MWCNTs scaffolds provides an alternative strategy for the regeneration of subchondral bone and lays a foundation for subsequent in vivo studies.
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spelling pubmed-98475192023-01-20 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone Cao, Yanyan Sun, Lei Liu, Zixian Shen, Zhizhong Jia, Wendan Hou, Peiyi Sang, Shengbo Regen Biomater Research Article Osteochondral defect caused by trauma or osteoarthritis exhibits a major challenge in clinical treatment with limited symptomatic effects at present. The regeneration and remodeling of subchondral bone play a positive effect on cartilage regeneration and further promotes the repair of osteochondral defects. Making use of the strengths of each preparation method, the combination of 3D printing and electrospinning is a promising method for designing and constructing multi-scale scaffolds that mimic the complexity and hierarchical structure of subchondral bone at the microscale and nanoscale, respectively. In this study, the 3D printed-electrospun poly(ɛ-caprolactone)/nano-hydroxyapatites/multi-walled carbon nanotubes (PCL/nHA/MWCNTs) scaffolds were successfully constructed by the combination of electrospinning and layer-by-layer 3D printing. The resulting dual-scale scaffold consisted of a dense layer of disordered nanospun fibers and a porous microscale 3D scaffold layer to support and promote the ingrowth of subchondral bone. Herein, the biomimetic PCL/nHA/MWCNTs scaffolds enhanced cell seeding efficiency and allowed for higher cell–cell interactions that supported the adhesion, proliferation, activity, morphology and subsequently improved the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Together, this study elucidates that the construction of 3D printed-electrospun PCL/nHA/MWCNTs scaffolds provides an alternative strategy for the regeneration of subchondral bone and lays a foundation for subsequent in vivo studies. Oxford University Press 2022-12-14 /pmc/articles/PMC9847519/ /pubmed/36683741 http://dx.doi.org/10.1093/rb/rbac104 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cao, Yanyan
Sun, Lei
Liu, Zixian
Shen, Zhizhong
Jia, Wendan
Hou, Peiyi
Sang, Shengbo
3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title_full 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title_fullStr 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title_full_unstemmed 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title_short 3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone
title_sort 3d printed-electrospun pcl/hydroxyapatite/mwcnts scaffolds for the repair of subchondral bone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847519/
https://www.ncbi.nlm.nih.gov/pubmed/36683741
http://dx.doi.org/10.1093/rb/rbac104
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