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Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis
AIMS: Viral myocarditis (VM) is an inflammatory pathology of the myocardium triggered by a viral infection that may cause sudden death or heart failure (HF), especially in the younger population. Current treatments only stabilize and improve cardiac function without resolving the underlying inflamma...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847559/ https://www.ncbi.nlm.nih.gov/pubmed/35426438 http://dx.doi.org/10.1093/cvr/cvac052 |
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author | Carai, Paolo González, Laura Florit Van Bruggen, Stijn Spalart, Valerie De Giorgio, Daria Geuens, Nadéche Martinod, Kimberly Jones, Elizabeth Anne Vincent Heymans, Stephane |
author_facet | Carai, Paolo González, Laura Florit Van Bruggen, Stijn Spalart, Valerie De Giorgio, Daria Geuens, Nadéche Martinod, Kimberly Jones, Elizabeth Anne Vincent Heymans, Stephane |
author_sort | Carai, Paolo |
collection | PubMed |
description | AIMS: Viral myocarditis (VM) is an inflammatory pathology of the myocardium triggered by a viral infection that may cause sudden death or heart failure (HF), especially in the younger population. Current treatments only stabilize and improve cardiac function without resolving the underlying inflammatory cause. The factors that induce VM to progress to HF are still uncertain, but neutrophils have been increasingly associated with the negative evolution of cardiac pathologies. The present study investigates the contribution of neutrophils to VM disease progression in different ways. METHODS AND RESULTS: In a coxsackievirus B3- (CVB3) induced mouse model of VM, neutrophils and neutrophil extracellular traps (NETs) were prominent in the acute phase of VM as revealed by enzyme-linked immunosorbent assay analysis and immunostaining. Anti-Ly6G-mediated neutrophil blockade starting at model induction decreased cardiac necrosis and leucocyte infiltration, preventing monocyte and Ly6C(High) pro-inflammatory macrophage recruitment. Furthermore, genetic peptidylarginine deiminase 4-dependent NET blockade reduced cardiac damage and leucocyte recruitment, significantly decreasing cardiac monocyte and macrophage presence. Depleting neutrophils with anti-Ly6G antibodies at 7 days post-infection, after the acute phase, did not decrease cardiac inflammation. CONCLUSION: Collectively, these results indicate that the repression of neutrophils and the related NET response in the acute phase of VM improves the pathological phenotype by reducing cardiac inflammation. |
format | Online Article Text |
id | pubmed-9847559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-98475592023-01-20 Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis Carai, Paolo González, Laura Florit Van Bruggen, Stijn Spalart, Valerie De Giorgio, Daria Geuens, Nadéche Martinod, Kimberly Jones, Elizabeth Anne Vincent Heymans, Stephane Cardiovasc Res Original Article AIMS: Viral myocarditis (VM) is an inflammatory pathology of the myocardium triggered by a viral infection that may cause sudden death or heart failure (HF), especially in the younger population. Current treatments only stabilize and improve cardiac function without resolving the underlying inflammatory cause. The factors that induce VM to progress to HF are still uncertain, but neutrophils have been increasingly associated with the negative evolution of cardiac pathologies. The present study investigates the contribution of neutrophils to VM disease progression in different ways. METHODS AND RESULTS: In a coxsackievirus B3- (CVB3) induced mouse model of VM, neutrophils and neutrophil extracellular traps (NETs) were prominent in the acute phase of VM as revealed by enzyme-linked immunosorbent assay analysis and immunostaining. Anti-Ly6G-mediated neutrophil blockade starting at model induction decreased cardiac necrosis and leucocyte infiltration, preventing monocyte and Ly6C(High) pro-inflammatory macrophage recruitment. Furthermore, genetic peptidylarginine deiminase 4-dependent NET blockade reduced cardiac damage and leucocyte recruitment, significantly decreasing cardiac monocyte and macrophage presence. Depleting neutrophils with anti-Ly6G antibodies at 7 days post-infection, after the acute phase, did not decrease cardiac inflammation. CONCLUSION: Collectively, these results indicate that the repression of neutrophils and the related NET response in the acute phase of VM improves the pathological phenotype by reducing cardiac inflammation. Oxford University Press 2022-04-15 /pmc/articles/PMC9847559/ /pubmed/35426438 http://dx.doi.org/10.1093/cvr/cvac052 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the European Society of Cardiology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Original Article Carai, Paolo González, Laura Florit Van Bruggen, Stijn Spalart, Valerie De Giorgio, Daria Geuens, Nadéche Martinod, Kimberly Jones, Elizabeth Anne Vincent Heymans, Stephane Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title | Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title_full | Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title_fullStr | Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title_full_unstemmed | Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title_short | Neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
title_sort | neutrophil inhibition improves acute inflammation in a murine model of viral myocarditis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847559/ https://www.ncbi.nlm.nih.gov/pubmed/35426438 http://dx.doi.org/10.1093/cvr/cvac052 |
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