Cargando…
Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have pred...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847903/ https://www.ncbi.nlm.nih.gov/pubmed/36652460 http://dx.doi.org/10.1371/journal.pone.0266207 |
_version_ | 1784871576633606144 |
---|---|
author | Pendexter, Casie A. Haque, Omar Mojoudi, Mohammadreza Maggipinto, Sarah Goutard, Marion Baicu, Simona Lellouch, Alexandre G. Markmann, James F. Brandacher, Gerald Yeh, Heidi Tessier, Shannon N. Cetrulo, Curtis Uygun, Korkut |
author_facet | Pendexter, Casie A. Haque, Omar Mojoudi, Mohammadreza Maggipinto, Sarah Goutard, Marion Baicu, Simona Lellouch, Alexandre G. Markmann, James F. Brandacher, Gerald Yeh, Heidi Tessier, Shannon N. Cetrulo, Curtis Uygun, Korkut |
author_sort | Pendexter, Casie A. |
collection | PubMed |
description | Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have predominately focused on hindlimb VCAs which does not translate anatomically to upper extremity transplantation, whereas the majority of clinical VCAs are upper extremity and hand transplants. This work details our optimization of rat forelimb VCA procurement and sub-normothermic machine perfusion (SNMP) protocols, with results in comparison to hindlimb perfusion with the same perfusion modality. Results indicate that compared to hindlimbs, rat forelimbs on machine perfusion mandate lower flow rates and higher acceptable maximum pressures. Additionally, low-flow forelimbs have less cellular damage than high-flow forelimbs based on oxygen uptake, edema, potassium levels, and histology through 2 hours of machine perfusion. These results are expected to inform future upper extremity VCA preservation studies. |
format | Online Article Text |
id | pubmed-9847903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98479032023-01-19 Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol Pendexter, Casie A. Haque, Omar Mojoudi, Mohammadreza Maggipinto, Sarah Goutard, Marion Baicu, Simona Lellouch, Alexandre G. Markmann, James F. Brandacher, Gerald Yeh, Heidi Tessier, Shannon N. Cetrulo, Curtis Uygun, Korkut PLoS One Research Article Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have predominately focused on hindlimb VCAs which does not translate anatomically to upper extremity transplantation, whereas the majority of clinical VCAs are upper extremity and hand transplants. This work details our optimization of rat forelimb VCA procurement and sub-normothermic machine perfusion (SNMP) protocols, with results in comparison to hindlimb perfusion with the same perfusion modality. Results indicate that compared to hindlimbs, rat forelimbs on machine perfusion mandate lower flow rates and higher acceptable maximum pressures. Additionally, low-flow forelimbs have less cellular damage than high-flow forelimbs based on oxygen uptake, edema, potassium levels, and histology through 2 hours of machine perfusion. These results are expected to inform future upper extremity VCA preservation studies. Public Library of Science 2023-01-18 /pmc/articles/PMC9847903/ /pubmed/36652460 http://dx.doi.org/10.1371/journal.pone.0266207 Text en © 2023 Pendexter et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Pendexter, Casie A. Haque, Omar Mojoudi, Mohammadreza Maggipinto, Sarah Goutard, Marion Baicu, Simona Lellouch, Alexandre G. Markmann, James F. Brandacher, Gerald Yeh, Heidi Tessier, Shannon N. Cetrulo, Curtis Uygun, Korkut Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title | Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title_full | Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title_fullStr | Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title_full_unstemmed | Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title_short | Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol |
title_sort | development of a rat forelimb vascularized composite allograft (vca) perfusion protocol |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847903/ https://www.ncbi.nlm.nih.gov/pubmed/36652460 http://dx.doi.org/10.1371/journal.pone.0266207 |
work_keys_str_mv | AT pendextercasiea developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT haqueomar developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT mojoudimohammadreza developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT maggipintosarah developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT goutardmarion developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT baicusimona developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT lellouchalexandreg developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT markmannjamesf developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT brandachergerald developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT yehheidi developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT tessiershannonn developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT cetrulocurtis developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol AT uygunkorkut developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol |