Cargando…

Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol

Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have pred...

Descripción completa

Detalles Bibliográficos
Autores principales: Pendexter, Casie A., Haque, Omar, Mojoudi, Mohammadreza, Maggipinto, Sarah, Goutard, Marion, Baicu, Simona, Lellouch, Alexandre G., Markmann, James F., Brandacher, Gerald, Yeh, Heidi, Tessier, Shannon N., Cetrulo, Curtis, Uygun, Korkut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847903/
https://www.ncbi.nlm.nih.gov/pubmed/36652460
http://dx.doi.org/10.1371/journal.pone.0266207
_version_ 1784871576633606144
author Pendexter, Casie A.
Haque, Omar
Mojoudi, Mohammadreza
Maggipinto, Sarah
Goutard, Marion
Baicu, Simona
Lellouch, Alexandre G.
Markmann, James F.
Brandacher, Gerald
Yeh, Heidi
Tessier, Shannon N.
Cetrulo, Curtis
Uygun, Korkut
author_facet Pendexter, Casie A.
Haque, Omar
Mojoudi, Mohammadreza
Maggipinto, Sarah
Goutard, Marion
Baicu, Simona
Lellouch, Alexandre G.
Markmann, James F.
Brandacher, Gerald
Yeh, Heidi
Tessier, Shannon N.
Cetrulo, Curtis
Uygun, Korkut
author_sort Pendexter, Casie A.
collection PubMed
description Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have predominately focused on hindlimb VCAs which does not translate anatomically to upper extremity transplantation, whereas the majority of clinical VCAs are upper extremity and hand transplants. This work details our optimization of rat forelimb VCA procurement and sub-normothermic machine perfusion (SNMP) protocols, with results in comparison to hindlimb perfusion with the same perfusion modality. Results indicate that compared to hindlimbs, rat forelimbs on machine perfusion mandate lower flow rates and higher acceptable maximum pressures. Additionally, low-flow forelimbs have less cellular damage than high-flow forelimbs based on oxygen uptake, edema, potassium levels, and histology through 2 hours of machine perfusion. These results are expected to inform future upper extremity VCA preservation studies.
format Online
Article
Text
id pubmed-9847903
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-98479032023-01-19 Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol Pendexter, Casie A. Haque, Omar Mojoudi, Mohammadreza Maggipinto, Sarah Goutard, Marion Baicu, Simona Lellouch, Alexandre G. Markmann, James F. Brandacher, Gerald Yeh, Heidi Tessier, Shannon N. Cetrulo, Curtis Uygun, Korkut PLoS One Research Article Vascularized composite allografts (VCAs) refer to en bloc heterogenous tissue that is transplanted to restore form and function after amputation or tissue loss. Rat limb VCA has emerged as a robust translational model to study the pathophysiology of these transplants. However, these models have predominately focused on hindlimb VCAs which does not translate anatomically to upper extremity transplantation, whereas the majority of clinical VCAs are upper extremity and hand transplants. This work details our optimization of rat forelimb VCA procurement and sub-normothermic machine perfusion (SNMP) protocols, with results in comparison to hindlimb perfusion with the same perfusion modality. Results indicate that compared to hindlimbs, rat forelimbs on machine perfusion mandate lower flow rates and higher acceptable maximum pressures. Additionally, low-flow forelimbs have less cellular damage than high-flow forelimbs based on oxygen uptake, edema, potassium levels, and histology through 2 hours of machine perfusion. These results are expected to inform future upper extremity VCA preservation studies. Public Library of Science 2023-01-18 /pmc/articles/PMC9847903/ /pubmed/36652460 http://dx.doi.org/10.1371/journal.pone.0266207 Text en © 2023 Pendexter et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pendexter, Casie A.
Haque, Omar
Mojoudi, Mohammadreza
Maggipinto, Sarah
Goutard, Marion
Baicu, Simona
Lellouch, Alexandre G.
Markmann, James F.
Brandacher, Gerald
Yeh, Heidi
Tessier, Shannon N.
Cetrulo, Curtis
Uygun, Korkut
Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title_full Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title_fullStr Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title_full_unstemmed Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title_short Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol
title_sort development of a rat forelimb vascularized composite allograft (vca) perfusion protocol
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847903/
https://www.ncbi.nlm.nih.gov/pubmed/36652460
http://dx.doi.org/10.1371/journal.pone.0266207
work_keys_str_mv AT pendextercasiea developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT haqueomar developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT mojoudimohammadreza developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT maggipintosarah developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT goutardmarion developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT baicusimona developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT lellouchalexandreg developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT markmannjamesf developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT brandachergerald developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT yehheidi developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT tessiershannonn developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT cetrulocurtis developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol
AT uygunkorkut developmentofaratforelimbvascularizedcompositeallograftvcaperfusionprotocol