Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)

DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor result...

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Detalles Bibliográficos
Autores principales: Vasilita, Cristina, Moser, Marina, Krogmann, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9848487/
https://www.ncbi.nlm.nih.gov/pubmed/36761532
http://dx.doi.org/10.3897/BDJ.10.e84860
Descripción
Sumario:DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor results. The inability to obtain barcodes for the majority of treated ceraphronoids halts progress on the taxonomy of this hyperdiverse parasitoid group. We here present a working protocol for the barcoding of ceraphronoid wasps which yields a first-time over 90% success rate.

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