Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)

DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor result...

Descripción completa

Detalles Bibliográficos
Autores principales: Vasilita, Cristina, Moser, Marina, Krogmann, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9848487/
https://www.ncbi.nlm.nih.gov/pubmed/36761532
http://dx.doi.org/10.3897/BDJ.10.e84860
_version_ 1784871722144497664
author Vasilita, Cristina
Moser, Marina
Krogmann, Lars
author_facet Vasilita, Cristina
Moser, Marina
Krogmann, Lars
author_sort Vasilita, Cristina
collection PubMed
description DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor results. The inability to obtain barcodes for the majority of treated ceraphronoids halts progress on the taxonomy of this hyperdiverse parasitoid group. We here present a working protocol for the barcoding of ceraphronoid wasps which yields a first-time over 90% success rate.
format Online
Article
Text
id pubmed-9848487
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Pensoft Publishers
record_format MEDLINE/PubMed
spelling pubmed-98484872023-02-08 Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera) Vasilita, Cristina Moser, Marina Krogmann, Lars Biodivers Data J Research Article DNA barcodes provide a reliable and efficient solution to resolving cryptic species complexes and accelerate species discoveries. The superfamily Ceraphronoidea (Hymenoptera) is a group of parasitoid wasps for which a barcoding approach could be of great help, if it were not for the very poor results. The inability to obtain barcodes for the majority of treated ceraphronoids halts progress on the taxonomy of this hyperdiverse parasitoid group. We here present a working protocol for the barcoding of ceraphronoid wasps which yields a first-time over 90% success rate. Pensoft Publishers 2022-06-30 /pmc/articles/PMC9848487/ /pubmed/36761532 http://dx.doi.org/10.3897/BDJ.10.e84860 Text en Cristina Vasilita, Marina Moser, Lars Krogmann https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vasilita, Cristina
Moser, Marina
Krogmann, Lars
Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title_full Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title_fullStr Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title_full_unstemmed Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title_short Mission possible: an optimised protocol for the unbarcodable Ceraphronoidea (Hymenoptera)
title_sort mission possible: an optimised protocol for the unbarcodable ceraphronoidea (hymenoptera)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9848487/
https://www.ncbi.nlm.nih.gov/pubmed/36761532
http://dx.doi.org/10.3897/BDJ.10.e84860
work_keys_str_mv AT vasilitacristina missionpossibleanoptimisedprotocolfortheunbarcodableceraphronoideahymenoptera
AT mosermarina missionpossibleanoptimisedprotocolfortheunbarcodableceraphronoideahymenoptera
AT krogmannlars missionpossibleanoptimisedprotocolfortheunbarcodableceraphronoideahymenoptera

Ejemplares similares