Cargando…

Evidence for further non-coding RNA genes in the fungal rDNA region

Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of severa...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosenblad, Magnus Alm, Larsson, Ellen, Walker, Arttapon, Thongklang, Naritsada, Wurzbacher, Christian, Nilsson, R. Henrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849065/
https://www.ncbi.nlm.nih.gov/pubmed/36760425
http://dx.doi.org/10.3897/mycokeys.90.84866
_version_ 1784871860105641984
author Rosenblad, Magnus Alm
Larsson, Ellen
Walker, Arttapon
Thongklang, Naritsada
Wurzbacher, Christian
Nilsson, R. Henrik
author_facet Rosenblad, Magnus Alm
Larsson, Ellen
Walker, Arttapon
Thongklang, Naritsada
Wurzbacher, Christian
Nilsson, R. Henrik
author_sort Rosenblad, Magnus Alm
collection PubMed
description Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of several species of three genera of ectomycorrhizal basidiomycetes prompted a more thorough bioinformatics search for additional ncRNA genes in the full fungal ribosomal operon. This study reports on the detection of three ncRNA genes hitherto not known from the fungal ribosomal region: nuclear RNase P RNA, RNase MRP RNA, and a possible snoRNA U14 in a total of five species of Auricularia and Inocybe. We verified their presence through resequencing of independent specimens. Two completed Auricularia genomes were found to lack these ncRNAs elsewhere than in the ribosomal operon, suggesting that these are functional genes. It seems clear that ncRNA genes play a larger role in fungal ribosomal genetics than hitherto thought.
format Online
Article
Text
id pubmed-9849065
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Pensoft Publishers
record_format MEDLINE/PubMed
spelling pubmed-98490652023-02-08 Evidence for further non-coding RNA genes in the fungal rDNA region Rosenblad, Magnus Alm Larsson, Ellen Walker, Arttapon Thongklang, Naritsada Wurzbacher, Christian Nilsson, R. Henrik MycoKeys Short Communication Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of several species of three genera of ectomycorrhizal basidiomycetes prompted a more thorough bioinformatics search for additional ncRNA genes in the full fungal ribosomal operon. This study reports on the detection of three ncRNA genes hitherto not known from the fungal ribosomal region: nuclear RNase P RNA, RNase MRP RNA, and a possible snoRNA U14 in a total of five species of Auricularia and Inocybe. We verified their presence through resequencing of independent specimens. Two completed Auricularia genomes were found to lack these ncRNAs elsewhere than in the ribosomal operon, suggesting that these are functional genes. It seems clear that ncRNA genes play a larger role in fungal ribosomal genetics than hitherto thought. Pensoft Publishers 2022-06-30 /pmc/articles/PMC9849065/ /pubmed/36760425 http://dx.doi.org/10.3897/mycokeys.90.84866 Text en Magnus Alm Rosenblad, Ellen Larsson, Arttapon Walker, Naritsada Thongklang, Christian Wurzbacher, R. Henrik Nilsson https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Short Communication
Rosenblad, Magnus Alm
Larsson, Ellen
Walker, Arttapon
Thongklang, Naritsada
Wurzbacher, Christian
Nilsson, R. Henrik
Evidence for further non-coding RNA genes in the fungal rDNA region
title Evidence for further non-coding RNA genes in the fungal rDNA region
title_full Evidence for further non-coding RNA genes in the fungal rDNA region
title_fullStr Evidence for further non-coding RNA genes in the fungal rDNA region
title_full_unstemmed Evidence for further non-coding RNA genes in the fungal rDNA region
title_short Evidence for further non-coding RNA genes in the fungal rDNA region
title_sort evidence for further non-coding rna genes in the fungal rdna region
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849065/
https://www.ncbi.nlm.nih.gov/pubmed/36760425
http://dx.doi.org/10.3897/mycokeys.90.84866
work_keys_str_mv AT rosenbladmagnusalm evidenceforfurthernoncodingrnagenesinthefungalrdnaregion
AT larssonellen evidenceforfurthernoncodingrnagenesinthefungalrdnaregion
AT walkerarttapon evidenceforfurthernoncodingrnagenesinthefungalrdnaregion
AT thongklangnaritsada evidenceforfurthernoncodingrnagenesinthefungalrdnaregion
AT wurzbacherchristian evidenceforfurthernoncodingrnagenesinthefungalrdnaregion
AT nilssonrhenrik evidenceforfurthernoncodingrnagenesinthefungalrdnaregion