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High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs

Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM) compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences—remain inaccessible by high-activity Cas9 proteins. Moreover, broadening PAM sequence compatibility through engineering can in...

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Detalles Bibliográficos
Autores principales:	Huang, Tony P., Heins, Zachary J., Miller, Shannon M., Wong, Brandon G., Balivada, Pallavi A., Wang, Tina, Khalil, Ahmad S., Liu, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group US 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849140/ https://www.ncbi.nlm.nih.gov/pubmed/36076084 http://dx.doi.org/10.1038/s41587-022-01410-2

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