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Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface

Biochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues influences embryonic development, however, is not extensively investigated. Using a comparative study of focal adhesion constituents between mouse embr...

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Autores principales: Zhang, Songjing, Chong, Lor Huai, Woon, Jessie Yong Xing, Chua, Theng Xuan, Cheruba, Elsie, Yip, Ai Kia, Li, Hoi-Yeung, Chiam, Keng-Hwee, Koh, Cheng-Gee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849324/
https://www.ncbi.nlm.nih.gov/pubmed/36653484
http://dx.doi.org/10.1038/s42003-023-04421-0
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author Zhang, Songjing
Chong, Lor Huai
Woon, Jessie Yong Xing
Chua, Theng Xuan
Cheruba, Elsie
Yip, Ai Kia
Li, Hoi-Yeung
Chiam, Keng-Hwee
Koh, Cheng-Gee
author_facet Zhang, Songjing
Chong, Lor Huai
Woon, Jessie Yong Xing
Chua, Theng Xuan
Cheruba, Elsie
Yip, Ai Kia
Li, Hoi-Yeung
Chiam, Keng-Hwee
Koh, Cheng-Gee
author_sort Zhang, Songjing
collection PubMed
description Biochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues influences embryonic development, however, is not extensively investigated. Using a comparative study of focal adhesion constituents between mouse embryonic stem cell (mESC) and their differentiated counterparts, we find while zyxin is lowly expressed in mESCs, its levels increase dramatically during early differentiation. Interestingly, overexpression of zyxin in mESCs suppresses Oct4 and Nanog. Using an integrative biochemical and biophysical approach, we demonstrate involvement of zyxin in regulating pluripotency through actin stress fibres and focal adhesions which are known to modulate cellular traction stress and facilitate substrate rigidity-sensing. YAP signaling is identified as an important biochemical effector of zyxin-induced mechanotransduction. These results provide insights into the role of zyxin in the integration of mechanical and biochemical cues for the regulation of embryonic stem cell fate.
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spelling pubmed-98493242023-01-20 Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface Zhang, Songjing Chong, Lor Huai Woon, Jessie Yong Xing Chua, Theng Xuan Cheruba, Elsie Yip, Ai Kia Li, Hoi-Yeung Chiam, Keng-Hwee Koh, Cheng-Gee Commun Biol Article Biochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues influences embryonic development, however, is not extensively investigated. Using a comparative study of focal adhesion constituents between mouse embryonic stem cell (mESC) and their differentiated counterparts, we find while zyxin is lowly expressed in mESCs, its levels increase dramatically during early differentiation. Interestingly, overexpression of zyxin in mESCs suppresses Oct4 and Nanog. Using an integrative biochemical and biophysical approach, we demonstrate involvement of zyxin in regulating pluripotency through actin stress fibres and focal adhesions which are known to modulate cellular traction stress and facilitate substrate rigidity-sensing. YAP signaling is identified as an important biochemical effector of zyxin-induced mechanotransduction. These results provide insights into the role of zyxin in the integration of mechanical and biochemical cues for the regulation of embryonic stem cell fate. Nature Publishing Group UK 2023-01-18 /pmc/articles/PMC9849324/ /pubmed/36653484 http://dx.doi.org/10.1038/s42003-023-04421-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Songjing
Chong, Lor Huai
Woon, Jessie Yong Xing
Chua, Theng Xuan
Cheruba, Elsie
Yip, Ai Kia
Li, Hoi-Yeung
Chiam, Keng-Hwee
Koh, Cheng-Gee
Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title_full Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title_fullStr Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title_full_unstemmed Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title_short Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
title_sort zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849324/
https://www.ncbi.nlm.nih.gov/pubmed/36653484
http://dx.doi.org/10.1038/s42003-023-04421-0
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