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Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients

Extracellular vesicles (EV) are a novel biomarker source for diagnosis and prognosis of cardiovascular disease. A protein comparison of plasma EVs in relation to blood plasma and atherosclerotic plaque has not been performed but would provide insight into the origin and content of biomarker sources...

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Autores principales: Verwer, Maarten C., Mekke, Joost, Timmerman, Nathalie, Waissi, Farahnaz, Boltjes, Arjan, Pasterkamp, Gerard, de Borst, Gert J., de Kleijn, Dominique P. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849473/
https://www.ncbi.nlm.nih.gov/pubmed/36653383
http://dx.doi.org/10.1038/s41598-023-27916-6
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author Verwer, Maarten C.
Mekke, Joost
Timmerman, Nathalie
Waissi, Farahnaz
Boltjes, Arjan
Pasterkamp, Gerard
de Borst, Gert J.
de Kleijn, Dominique P. V.
author_facet Verwer, Maarten C.
Mekke, Joost
Timmerman, Nathalie
Waissi, Farahnaz
Boltjes, Arjan
Pasterkamp, Gerard
de Borst, Gert J.
de Kleijn, Dominique P. V.
author_sort Verwer, Maarten C.
collection PubMed
description Extracellular vesicles (EV) are a novel biomarker source for diagnosis and prognosis of cardiovascular disease. A protein comparison of plasma EVs in relation to blood plasma and atherosclerotic plaque has not been performed but would provide insight into the origin and content of biomarker sources and their association with atherosclerotic progression. Using samples of 88 carotid endarterectomy patients in the Athero-Express, 92 proteins (Olink Cardiovascular III panel) were measured in citrate plasma, plasma derived LDL-EVs and atherosclerotic plaque. Proteins were correlated between sources and were related to pre-operative stroke and 3-year major adverse cardiovascular events (MACE). Plasma and EV proteins correlated moderately on average, but with substantial variability. Both showed little correlation with plaque, suggesting that these circulating biomarkers may not originate from the latter. Plaque (n = 17) contained most differentially-expressed proteins in patients with stroke, opposed to EVs (n = 6) and plasma (n = 5). In contrast, EVs contained most differentially-expressed proteins for MACE (n = 21) compared to plasma (n = 9) and plaque (n = 1). EVs appear to provide additional information about severity and progression of systemic atherosclerosis than can be obtained from plasma or atherosclerotic plaque.
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spelling pubmed-98494732023-01-20 Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients Verwer, Maarten C. Mekke, Joost Timmerman, Nathalie Waissi, Farahnaz Boltjes, Arjan Pasterkamp, Gerard de Borst, Gert J. de Kleijn, Dominique P. V. Sci Rep Article Extracellular vesicles (EV) are a novel biomarker source for diagnosis and prognosis of cardiovascular disease. A protein comparison of plasma EVs in relation to blood plasma and atherosclerotic plaque has not been performed but would provide insight into the origin and content of biomarker sources and their association with atherosclerotic progression. Using samples of 88 carotid endarterectomy patients in the Athero-Express, 92 proteins (Olink Cardiovascular III panel) were measured in citrate plasma, plasma derived LDL-EVs and atherosclerotic plaque. Proteins were correlated between sources and were related to pre-operative stroke and 3-year major adverse cardiovascular events (MACE). Plasma and EV proteins correlated moderately on average, but with substantial variability. Both showed little correlation with plaque, suggesting that these circulating biomarkers may not originate from the latter. Plaque (n = 17) contained most differentially-expressed proteins in patients with stroke, opposed to EVs (n = 6) and plasma (n = 5). In contrast, EVs contained most differentially-expressed proteins for MACE (n = 21) compared to plasma (n = 9) and plaque (n = 1). EVs appear to provide additional information about severity and progression of systemic atherosclerosis than can be obtained from plasma or atherosclerotic plaque. Nature Publishing Group UK 2023-01-18 /pmc/articles/PMC9849473/ /pubmed/36653383 http://dx.doi.org/10.1038/s41598-023-27916-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Verwer, Maarten C.
Mekke, Joost
Timmerman, Nathalie
Waissi, Farahnaz
Boltjes, Arjan
Pasterkamp, Gerard
de Borst, Gert J.
de Kleijn, Dominique P. V.
Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title_full Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title_fullStr Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title_full_unstemmed Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title_short Comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of MACE in CEA patients
title_sort comparison of cardiovascular biomarker expression in extracellular vesicles, plasma and carotid plaque for the prediction of mace in cea patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849473/
https://www.ncbi.nlm.nih.gov/pubmed/36653383
http://dx.doi.org/10.1038/s41598-023-27916-6
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