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Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract

INTRODUCTION: Diabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries. METHODS: The plant under investigation in the current study Phr...

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Autores principales: Khodeer, Dina M., Nasr, Ali M., Swidan, Shady A., Shabayek, Sarah, Khinkar, Roaa M., Aldurdunji, Mohammed M., Ramadan, Maryam A., Badr, Jihan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849905/
https://www.ncbi.nlm.nih.gov/pubmed/36687608
http://dx.doi.org/10.3389/fmicb.2022.1078061
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author Khodeer, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Shabayek, Sarah
Khinkar, Roaa M.
Aldurdunji, Mohammed M.
Ramadan, Maryam A.
Badr, Jihan M.
author_facet Khodeer, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Shabayek, Sarah
Khinkar, Roaa M.
Aldurdunji, Mohammed M.
Ramadan, Maryam A.
Badr, Jihan M.
author_sort Khodeer, Dina M.
collection PubMed
description INTRODUCTION: Diabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries. METHODS: The plant under investigation in the current study Phragmanthera austroarabica A. G. Mill and J. A. Nyberg is traditionally used in Saudi Arabia for the treatment of diabetes mellitus. The methanolic extract (200 mg/kg) of the plant and pure gallic acid (40 mg/kg), a major metabolite of the plant, as well as their silver nanoparticle formulae (AgNPs) were evaluated for their antidiabetic activity. RESULTS AND DISCUSSION: The results showed a decrease in body fat, obesity, an improvement in lipid profiles, normalization of hyperglycemia, insulin resistance, and hyperinsulinemia, and an improvement in liver tissue structure and function. However, the results obtained from AgNPs for both extract and the pure gallic acid were better in most measured parameters. Additionally, the activity of both the crude extract of the plant and its AgNPs were evaluated against a number of gram-positive, gram-negative bacteria and fungi. Although the activity of the crude extract ranged from moderate to weak or even non-active, the AgNPs of the plant extract clearly enhanced the antimicrobial activity. AgNPs of the extract demonstrated remarkable activity, especially against the Gram-negative pathogens Proteus vulgaris (MIC 2.5 μg/ml) and Pseudomonas aeruginosa (MIC 5 μg/ml). Furthermore, a promising antimicrobial activity was shown against the Gram-positive pathogen Streptococcus mutants (MIC 1.25 μg/ml).
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spelling pubmed-98499052023-01-20 Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract Khodeer, Dina M. Nasr, Ali M. Swidan, Shady A. Shabayek, Sarah Khinkar, Roaa M. Aldurdunji, Mohammed M. Ramadan, Maryam A. Badr, Jihan M. Front Microbiol Microbiology INTRODUCTION: Diabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries. METHODS: The plant under investigation in the current study Phragmanthera austroarabica A. G. Mill and J. A. Nyberg is traditionally used in Saudi Arabia for the treatment of diabetes mellitus. The methanolic extract (200 mg/kg) of the plant and pure gallic acid (40 mg/kg), a major metabolite of the plant, as well as their silver nanoparticle formulae (AgNPs) were evaluated for their antidiabetic activity. RESULTS AND DISCUSSION: The results showed a decrease in body fat, obesity, an improvement in lipid profiles, normalization of hyperglycemia, insulin resistance, and hyperinsulinemia, and an improvement in liver tissue structure and function. However, the results obtained from AgNPs for both extract and the pure gallic acid were better in most measured parameters. Additionally, the activity of both the crude extract of the plant and its AgNPs were evaluated against a number of gram-positive, gram-negative bacteria and fungi. Although the activity of the crude extract ranged from moderate to weak or even non-active, the AgNPs of the plant extract clearly enhanced the antimicrobial activity. AgNPs of the extract demonstrated remarkable activity, especially against the Gram-negative pathogens Proteus vulgaris (MIC 2.5 μg/ml) and Pseudomonas aeruginosa (MIC 5 μg/ml). Furthermore, a promising antimicrobial activity was shown against the Gram-positive pathogen Streptococcus mutants (MIC 1.25 μg/ml). Frontiers Media S.A. 2023-01-05 /pmc/articles/PMC9849905/ /pubmed/36687608 http://dx.doi.org/10.3389/fmicb.2022.1078061 Text en Copyright © 2023 Khodeer, Nasr, Swidan, Shabayek, Khinkar, Aldurdunji, Ramadan and Badr. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Khodeer, Dina M.
Nasr, Ali M.
Swidan, Shady A.
Shabayek, Sarah
Khinkar, Roaa M.
Aldurdunji, Mohammed M.
Ramadan, Maryam A.
Badr, Jihan M.
Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title_full Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title_fullStr Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title_full_unstemmed Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title_short Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract
title_sort characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using phragmanthera austroarabica a. g. mill and j. a. nyberg extract
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849905/
https://www.ncbi.nlm.nih.gov/pubmed/36687608
http://dx.doi.org/10.3389/fmicb.2022.1078061
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