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Lost in transduction: Critical considerations when using viral vectors
The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential c...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849944/ https://www.ncbi.nlm.nih.gov/pubmed/36684429 http://dx.doi.org/10.3389/fcell.2022.1080265 |
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author | Hines, William C. Hines, William C. |
author_facet | Hines, William C. Hines, William C. |
author_sort | Hines, William C. |
collection | PubMed |
description | The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory. |
format | Online Article Text |
id | pubmed-9849944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98499442023-01-20 Lost in transduction: Critical considerations when using viral vectors Hines, William C. Hines, William C. Front Cell Dev Biol Cell and Developmental Biology The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory. Frontiers Media S.A. 2023-01-05 /pmc/articles/PMC9849944/ /pubmed/36684429 http://dx.doi.org/10.3389/fcell.2022.1080265 Text en Copyright © 2023 Hines and Hines. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Hines, William C. Hines, William C. Lost in transduction: Critical considerations when using viral vectors |
title | Lost in transduction: Critical considerations when using viral vectors |
title_full | Lost in transduction: Critical considerations when using viral vectors |
title_fullStr | Lost in transduction: Critical considerations when using viral vectors |
title_full_unstemmed | Lost in transduction: Critical considerations when using viral vectors |
title_short | Lost in transduction: Critical considerations when using viral vectors |
title_sort | lost in transduction: critical considerations when using viral vectors |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849944/ https://www.ncbi.nlm.nih.gov/pubmed/36684429 http://dx.doi.org/10.3389/fcell.2022.1080265 |
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