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Lost in transduction: Critical considerations when using viral vectors

The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential c...

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Detalles Bibliográficos
Autor principal: Hines, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849944/
https://www.ncbi.nlm.nih.gov/pubmed/36684429
http://dx.doi.org/10.3389/fcell.2022.1080265
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author Hines, William C.
Hines, William C.
author_facet Hines, William C.
Hines, William C.
author_sort Hines, William C.
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description The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory.
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spelling pubmed-98499442023-01-20 Lost in transduction: Critical considerations when using viral vectors Hines, William C. Hines, William C. Front Cell Dev Biol Cell and Developmental Biology The application of retroviral vectors in the laboratory requires considerations that often go overlooked but are often easy to circumvent. Here, we discuss the relationship between the observed transduction efficiency of a cell population and per-cell viral insertions—and describe how differential cell-type susceptibilities can confound results. We consider the math underlying this problem and review an alternative approach to the commonly used “multiplicity of infection” (MOI) method of titering and using viral vectors in the biomedical research laboratory. Frontiers Media S.A. 2023-01-05 /pmc/articles/PMC9849944/ /pubmed/36684429 http://dx.doi.org/10.3389/fcell.2022.1080265 Text en Copyright © 2023 Hines and Hines. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Hines, William C.
Hines, William C.
Lost in transduction: Critical considerations when using viral vectors
title Lost in transduction: Critical considerations when using viral vectors
title_full Lost in transduction: Critical considerations when using viral vectors
title_fullStr Lost in transduction: Critical considerations when using viral vectors
title_full_unstemmed Lost in transduction: Critical considerations when using viral vectors
title_short Lost in transduction: Critical considerations when using viral vectors
title_sort lost in transduction: critical considerations when using viral vectors
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849944/
https://www.ncbi.nlm.nih.gov/pubmed/36684429
http://dx.doi.org/10.3389/fcell.2022.1080265
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