Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo

Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation. In this protocol, we describe the use of cultured mESCs...

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Detalles Bibliográficos
Autores principales: Khateb, Mamduh, Feng, Xuesong, Dell’Orso, Stefania, Sartorelli, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850182/
https://www.ncbi.nlm.nih.gov/pubmed/36607809
http://dx.doi.org/10.1016/j.xpro.2022.102001
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author Khateb, Mamduh
Feng, Xuesong
Dell’Orso, Stefania
Sartorelli, Vittorio
author_facet Khateb, Mamduh
Feng, Xuesong
Dell’Orso, Stefania
Sartorelli, Vittorio
author_sort Khateb, Mamduh
collection PubMed
description Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation. In this protocol, we describe the use of cultured mESCs to generate presomitic-like mesoderm cells undergoing further specification towards myogenic progenitors (MPs). Further, we describe here a procedure to obtain, dissect, and fluorescence-activated cell sorting (FACS)-isolate somitic cells from genetically labeled Pax7(+/Cre); Rosa26(YFP/+) embryos. For complete details on the use and execution of this protocol, please refer to Khateb et al.(1)
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spelling pubmed-98501822023-01-20 Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo Khateb, Mamduh Feng, Xuesong Dell’Orso, Stefania Sartorelli, Vittorio STAR Protoc Protocol Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation. In this protocol, we describe the use of cultured mESCs to generate presomitic-like mesoderm cells undergoing further specification towards myogenic progenitors (MPs). Further, we describe here a procedure to obtain, dissect, and fluorescence-activated cell sorting (FACS)-isolate somitic cells from genetically labeled Pax7(+/Cre); Rosa26(YFP/+) embryos. For complete details on the use and execution of this protocol, please refer to Khateb et al.(1) Elsevier 2023-01-05 /pmc/articles/PMC9850182/ /pubmed/36607809 http://dx.doi.org/10.1016/j.xpro.2022.102001 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Khateb, Mamduh
Feng, Xuesong
Dell’Orso, Stefania
Sartorelli, Vittorio
Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title_full Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title_fullStr Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title_full_unstemmed Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title_short Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
title_sort protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850182/
https://www.ncbi.nlm.nih.gov/pubmed/36607809
http://dx.doi.org/10.1016/j.xpro.2022.102001
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