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Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats

BACKGROUND: Zhijing Powder (ZJP) is a traditional Chinese medicine containing two kinds of Chinese medicine. Those studies analyze the molecular mechanism of ZJP in treating hypertension through network pharmacology, combined with animal experiments. METHODS: First, the effective ingredients and pot...

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Detalles Bibliográficos
Autores principales: Wang, Yue, Zhang, Pengfei, Li, Hao, Chen, Pingping, Zhang, Xia, Wang, Bin, Zhang, Mingquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850196/
https://www.ncbi.nlm.nih.gov/pubmed/36685421
http://dx.doi.org/10.1016/j.heliyon.2022.e12777
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author Wang, Yue
Zhang, Pengfei
Li, Hao
Chen, Pingping
Zhang, Xia
Wang, Bin
Zhang, Mingquan
author_facet Wang, Yue
Zhang, Pengfei
Li, Hao
Chen, Pingping
Zhang, Xia
Wang, Bin
Zhang, Mingquan
author_sort Wang, Yue
collection PubMed
description BACKGROUND: Zhijing Powder (ZJP) is a traditional Chinese medicine containing two kinds of Chinese medicine. Those studies analyze the molecular mechanism of ZJP in treating hypertension through network pharmacology, combined with animal experiments. METHODS: First, the effective ingredients and potential targets of the drug were obtained through drug databases, while the targets of disease obtained through disease target databases. The potential targets, cellular bioanalysis and signaling pathways were found in some platforms by analyzing collected targets. Further experiments were conducted to verify the effect and mechanism of drugs on cold and high salt in an induced-hypertension rat model. RESULTS: There are 17 effective components of centipedes and 10 of scorpions, with 464 drug targets obtained after screening. A total of 1263 hypertension targets were obtained after screening and integration, resulting in a protein-protein interaction network (PPI) with 145 points and 1310 edges. Gene ontology (GO) analysis shows that blood circulation regulation and activation of G protein-coupled receptors are mainly biological processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis shows that neuroactive ligand-receptor interaction, calcium signaling pathways, PI3K-AKT signaling pathways are the most abundant gene-enriched pathway. Animal experiments indicated that ZJP can reduce blood pressure (BP), affect expression of the PI3K-AKT signaling pathway, and improve oxidative stress in the body. CONCLUSION: ZJP ameliorates oxidative stress and reduces BP in hypertensive rats caused by cold stimuli and high salt, revealing its effect on the expression of the PI3K/AKT signaling pathway in the rat aorta.
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spelling pubmed-98501962023-01-20 Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats Wang, Yue Zhang, Pengfei Li, Hao Chen, Pingping Zhang, Xia Wang, Bin Zhang, Mingquan Heliyon Research Article BACKGROUND: Zhijing Powder (ZJP) is a traditional Chinese medicine containing two kinds of Chinese medicine. Those studies analyze the molecular mechanism of ZJP in treating hypertension through network pharmacology, combined with animal experiments. METHODS: First, the effective ingredients and potential targets of the drug were obtained through drug databases, while the targets of disease obtained through disease target databases. The potential targets, cellular bioanalysis and signaling pathways were found in some platforms by analyzing collected targets. Further experiments were conducted to verify the effect and mechanism of drugs on cold and high salt in an induced-hypertension rat model. RESULTS: There are 17 effective components of centipedes and 10 of scorpions, with 464 drug targets obtained after screening. A total of 1263 hypertension targets were obtained after screening and integration, resulting in a protein-protein interaction network (PPI) with 145 points and 1310 edges. Gene ontology (GO) analysis shows that blood circulation regulation and activation of G protein-coupled receptors are mainly biological processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis shows that neuroactive ligand-receptor interaction, calcium signaling pathways, PI3K-AKT signaling pathways are the most abundant gene-enriched pathway. Animal experiments indicated that ZJP can reduce blood pressure (BP), affect expression of the PI3K-AKT signaling pathway, and improve oxidative stress in the body. CONCLUSION: ZJP ameliorates oxidative stress and reduces BP in hypertensive rats caused by cold stimuli and high salt, revealing its effect on the expression of the PI3K/AKT signaling pathway in the rat aorta. Elsevier 2023-01-02 /pmc/articles/PMC9850196/ /pubmed/36685421 http://dx.doi.org/10.1016/j.heliyon.2022.e12777 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Wang, Yue
Zhang, Pengfei
Li, Hao
Chen, Pingping
Zhang, Xia
Wang, Bin
Zhang, Mingquan
Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title_full Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title_fullStr Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title_full_unstemmed Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title_short Zhijing powder manages blood pressure by regulating PI3K/AKT signal pathway in hypertensive rats
title_sort zhijing powder manages blood pressure by regulating pi3k/akt signal pathway in hypertensive rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850196/
https://www.ncbi.nlm.nih.gov/pubmed/36685421
http://dx.doi.org/10.1016/j.heliyon.2022.e12777
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