Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

BACKGROUND: Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system has proven to be an important and versatile tool for functional genomics, tissue-...

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Detalles Bibliográficos
Autores principales: Zhang, Ke, Liu, Shanhe, Fu, Yunze, Wang, Zixuan, Yang, Xiubo, Li, Wenjing, Zhang, Caihua, Zhang, Dongmei, Li, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850602/
https://www.ncbi.nlm.nih.gov/pubmed/36653863
http://dx.doi.org/10.1186/s13007-023-00983-6

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