Cargando…
The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significanc...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850894/ https://www.ncbi.nlm.nih.gov/pubmed/36412334 http://dx.doi.org/10.4103/aam.aam_87_21 |
_version_ | 1784872284942499840 |
---|---|
author | Tiwana, Kanwardeep Kaur Kaur, Mohanvir Goyal, Shaina Bhandhari, Lachhima |
author_facet | Tiwana, Kanwardeep Kaur Kaur, Mohanvir Goyal, Shaina Bhandhari, Lachhima |
author_sort | Tiwana, Kanwardeep Kaur |
collection | PubMed |
description | AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significance (ASC-US), abnormal squamous cells cannot exclude high-grade squamous intraepithelial lesion (HSIL) (ASC-H), low-grade squamous intraepithelial lesion (LSIL), HSIL, and invasive carcinoma (IC) and to assess the role of MN scoring as a biomarker for predicting risk of carcinoma. MATERIALS AND METHODS: A total of 1000 conventional cervical smears stained with Papanicolaou (Pap) stain, comprising unsatisfactory for evaluation (86), NILM (140), inflammatory (696), ASC-US (23), ASC-H (16), LSIL (18), HSIL (15), and IC (6) were studied independently by two pathologists, and the number of MN cells per 1000 epithelial cells in high-power (×400) and oil immersion (×1000) was counted and expressed as MN score per 1000 cells. RESULTS: The mean MN score ± standard deviation was found to be 0.99 ± 0.744 in NILM cases, 0.67 ± 0.782 in inflammatory cases, 1.57 ± 0.507 in ASC-US cases, 1.63 ± 0.50 in ASC-H cases, 1.56 ± 0.511 in LSIL cases, 2.47 ± 0.516 in HSIL cases, and 3.0 ± 0.00 in IC cases. A step-wise increase was observed in MN score from inflammatory to IC categories. CONCLUSIONS: MN score is a reliable and easy test that can be used in conjunction with routine cervical PAP to assess the risk of malignant transformation in the uterine cervix as a biomarker for predicting the risk of carcinoma. |
format | Online Article Text |
id | pubmed-9850894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-98508942023-01-20 The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study Tiwana, Kanwardeep Kaur Kaur, Mohanvir Goyal, Shaina Bhandhari, Lachhima Ann Afr Med Original Article AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significance (ASC-US), abnormal squamous cells cannot exclude high-grade squamous intraepithelial lesion (HSIL) (ASC-H), low-grade squamous intraepithelial lesion (LSIL), HSIL, and invasive carcinoma (IC) and to assess the role of MN scoring as a biomarker for predicting risk of carcinoma. MATERIALS AND METHODS: A total of 1000 conventional cervical smears stained with Papanicolaou (Pap) stain, comprising unsatisfactory for evaluation (86), NILM (140), inflammatory (696), ASC-US (23), ASC-H (16), LSIL (18), HSIL (15), and IC (6) were studied independently by two pathologists, and the number of MN cells per 1000 epithelial cells in high-power (×400) and oil immersion (×1000) was counted and expressed as MN score per 1000 cells. RESULTS: The mean MN score ± standard deviation was found to be 0.99 ± 0.744 in NILM cases, 0.67 ± 0.782 in inflammatory cases, 1.57 ± 0.507 in ASC-US cases, 1.63 ± 0.50 in ASC-H cases, 1.56 ± 0.511 in LSIL cases, 2.47 ± 0.516 in HSIL cases, and 3.0 ± 0.00 in IC cases. A step-wise increase was observed in MN score from inflammatory to IC categories. CONCLUSIONS: MN score is a reliable and easy test that can be used in conjunction with routine cervical PAP to assess the risk of malignant transformation in the uterine cervix as a biomarker for predicting the risk of carcinoma. Wolters Kluwer - Medknow 2022 2022-11-16 /pmc/articles/PMC9850894/ /pubmed/36412334 http://dx.doi.org/10.4103/aam.aam_87_21 Text en Copyright: © 2022 Annals of African Medicine https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Tiwana, Kanwardeep Kaur Kaur, Mohanvir Goyal, Shaina Bhandhari, Lachhima The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title | The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title_full | The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title_fullStr | The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title_full_unstemmed | The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title_short | The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study |
title_sort | role of micronucleus scoring in cervical papanicolaou smears: a 1-year study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850894/ https://www.ncbi.nlm.nih.gov/pubmed/36412334 http://dx.doi.org/10.4103/aam.aam_87_21 |
work_keys_str_mv | AT tiwanakanwardeepkaur theroleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT kaurmohanvir theroleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT goyalshaina theroleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT bhandharilachhima theroleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT tiwanakanwardeepkaur roleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT kaurmohanvir roleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT goyalshaina roleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy AT bhandharilachhima roleofmicronucleusscoringincervicalpapanicolaousmearsa1yearstudy |