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The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study

AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significanc...

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Autores principales: Tiwana, Kanwardeep Kaur, Kaur, Mohanvir, Goyal, Shaina, Bhandhari, Lachhima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850894/
https://www.ncbi.nlm.nih.gov/pubmed/36412334
http://dx.doi.org/10.4103/aam.aam_87_21
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author Tiwana, Kanwardeep Kaur
Kaur, Mohanvir
Goyal, Shaina
Bhandhari, Lachhima
author_facet Tiwana, Kanwardeep Kaur
Kaur, Mohanvir
Goyal, Shaina
Bhandhari, Lachhima
author_sort Tiwana, Kanwardeep Kaur
collection PubMed
description AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significance (ASC-US), abnormal squamous cells cannot exclude high-grade squamous intraepithelial lesion (HSIL) (ASC-H), low-grade squamous intraepithelial lesion (LSIL), HSIL, and invasive carcinoma (IC) and to assess the role of MN scoring as a biomarker for predicting risk of carcinoma. MATERIALS AND METHODS: A total of 1000 conventional cervical smears stained with Papanicolaou (Pap) stain, comprising unsatisfactory for evaluation (86), NILM (140), inflammatory (696), ASC-US (23), ASC-H (16), LSIL (18), HSIL (15), and IC (6) were studied independently by two pathologists, and the number of MN cells per 1000 epithelial cells in high-power (×400) and oil immersion (×1000) was counted and expressed as MN score per 1000 cells. RESULTS: The mean MN score ± standard deviation was found to be 0.99 ± 0.744 in NILM cases, 0.67 ± 0.782 in inflammatory cases, 1.57 ± 0.507 in ASC-US cases, 1.63 ± 0.50 in ASC-H cases, 1.56 ± 0.511 in LSIL cases, 2.47 ± 0.516 in HSIL cases, and 3.0 ± 0.00 in IC cases. A step-wise increase was observed in MN score from inflammatory to IC categories. CONCLUSIONS: MN score is a reliable and easy test that can be used in conjunction with routine cervical PAP to assess the risk of malignant transformation in the uterine cervix as a biomarker for predicting the risk of carcinoma.
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spelling pubmed-98508942023-01-20 The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study Tiwana, Kanwardeep Kaur Kaur, Mohanvir Goyal, Shaina Bhandhari, Lachhima Ann Afr Med Original Article AIMS AND OBJECTIVES: To compare the micronucleus (MN) score in all the major diagnostic categories as per “The Bethesda System for Reporting Cervical Cytology” 2014 including negative for intraepithelial lesions and malignancy (NILM), inflammatory, abnormal squamous cells of undetermined significance (ASC-US), abnormal squamous cells cannot exclude high-grade squamous intraepithelial lesion (HSIL) (ASC-H), low-grade squamous intraepithelial lesion (LSIL), HSIL, and invasive carcinoma (IC) and to assess the role of MN scoring as a biomarker for predicting risk of carcinoma. MATERIALS AND METHODS: A total of 1000 conventional cervical smears stained with Papanicolaou (Pap) stain, comprising unsatisfactory for evaluation (86), NILM (140), inflammatory (696), ASC-US (23), ASC-H (16), LSIL (18), HSIL (15), and IC (6) were studied independently by two pathologists, and the number of MN cells per 1000 epithelial cells in high-power (×400) and oil immersion (×1000) was counted and expressed as MN score per 1000 cells. RESULTS: The mean MN score ± standard deviation was found to be 0.99 ± 0.744 in NILM cases, 0.67 ± 0.782 in inflammatory cases, 1.57 ± 0.507 in ASC-US cases, 1.63 ± 0.50 in ASC-H cases, 1.56 ± 0.511 in LSIL cases, 2.47 ± 0.516 in HSIL cases, and 3.0 ± 0.00 in IC cases. A step-wise increase was observed in MN score from inflammatory to IC categories. CONCLUSIONS: MN score is a reliable and easy test that can be used in conjunction with routine cervical PAP to assess the risk of malignant transformation in the uterine cervix as a biomarker for predicting the risk of carcinoma. Wolters Kluwer - Medknow 2022 2022-11-16 /pmc/articles/PMC9850894/ /pubmed/36412334 http://dx.doi.org/10.4103/aam.aam_87_21 Text en Copyright: © 2022 Annals of African Medicine https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Tiwana, Kanwardeep Kaur
Kaur, Mohanvir
Goyal, Shaina
Bhandhari, Lachhima
The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title_full The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title_fullStr The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title_full_unstemmed The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title_short The Role of Micronucleus Scoring in Cervical Papanicolaou Smears: A 1-Year Study
title_sort role of micronucleus scoring in cervical papanicolaou smears: a 1-year study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850894/
https://www.ncbi.nlm.nih.gov/pubmed/36412334
http://dx.doi.org/10.4103/aam.aam_87_21
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