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High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin

BACKGROUND: Communication between oocytes and granulosa cells ultimately dictate follicle development or atresia. Melatonin is also involved in follicle development. This study aimed to investigate the effects of melatonin and its receptor antagonists on hormone secretion, as well as gene expression...

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Autores principales: Liu, Wenju, Chen, Zhihao, Li, Rui, Zheng, Menghao, Pang, Xunsheng, Wen, Aiyou, Yang, Bing, Wang, Shujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851050/
https://www.ncbi.nlm.nih.gov/pubmed/36684672
http://dx.doi.org/10.7717/peerj.14612
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author Liu, Wenju
Chen, Zhihao
Li, Rui
Zheng, Menghao
Pang, Xunsheng
Wen, Aiyou
Yang, Bing
Wang, Shujuan
author_facet Liu, Wenju
Chen, Zhihao
Li, Rui
Zheng, Menghao
Pang, Xunsheng
Wen, Aiyou
Yang, Bing
Wang, Shujuan
author_sort Liu, Wenju
collection PubMed
description BACKGROUND: Communication between oocytes and granulosa cells ultimately dictate follicle development or atresia. Melatonin is also involved in follicle development. This study aimed to investigate the effects of melatonin and its receptor antagonists on hormone secretion, as well as gene expression related to hormone synthesis, TGF-β superfamily, and follicle development in bovine granulosa cells, and assess the effects of melatonin in the presence of 4-P-PDOT and luzindole. METHODS: Bovine ovaries were collected from a local abattoir and follicular fluid (follicle diameter 5–8 mm) was collected for granulosa cell isolation and culture. Granulosa cells and culture medium were collected 48 h after treatment with melatonin at high dose concentrations (10(−5) M) and low dose concentrations (10(−9) M) in the absence/presence of 4-P-PDOT and luzindole (10(−5) M or 10(−9) M). Furthermore, the expression level of genes related to hormonal synthesis (CYP11A1, CYP19A1, StAR, and RUNX2), TGF-β superfamily (BMP6, INHA, INHBA, INHBB, and TGFBR3), and development (EGFR, DNMT1A, and FSHR) were detected in each experimental group by real-time quantitative PCR. In addition, the level of hormones in culture medium were detected using ELISA. RESULTS: Both 10(−5) M and 10(−9) M melatonin doses promoted the secretion of inhibin A and progesterone without affecting the production of inhibin B and estradiol. In addition, both promoted the gene expression of INHA, StAR, RUNX2, TGFBR3, EGFR, and DNMT1A, and inhibited the expression of BMP6, INHBB, CYP11A1, CYP19A1, and FSHR. When combined with different doses of 4-P-PDOT and luzindole, they exhibited different effects on the secretion of inhibin B, estradiol, inhibin A, and progesterone, and the expression of CYP19A1, RUNX2, BMP6, INHBB, EGFR, and DNMT1A induced by melatonin. CONCLUSION: High and low dose melatonin receptor antagonists exhibited different effects in regulating hormone secretion and the expression of various genes in response to melatonin. Therefore, concentration effects must be considered when using luzindole or 4-P-PDOT.
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spelling pubmed-98510502023-01-20 High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin Liu, Wenju Chen, Zhihao Li, Rui Zheng, Menghao Pang, Xunsheng Wen, Aiyou Yang, Bing Wang, Shujuan PeerJ Biochemistry BACKGROUND: Communication between oocytes and granulosa cells ultimately dictate follicle development or atresia. Melatonin is also involved in follicle development. This study aimed to investigate the effects of melatonin and its receptor antagonists on hormone secretion, as well as gene expression related to hormone synthesis, TGF-β superfamily, and follicle development in bovine granulosa cells, and assess the effects of melatonin in the presence of 4-P-PDOT and luzindole. METHODS: Bovine ovaries were collected from a local abattoir and follicular fluid (follicle diameter 5–8 mm) was collected for granulosa cell isolation and culture. Granulosa cells and culture medium were collected 48 h after treatment with melatonin at high dose concentrations (10(−5) M) and low dose concentrations (10(−9) M) in the absence/presence of 4-P-PDOT and luzindole (10(−5) M or 10(−9) M). Furthermore, the expression level of genes related to hormonal synthesis (CYP11A1, CYP19A1, StAR, and RUNX2), TGF-β superfamily (BMP6, INHA, INHBA, INHBB, and TGFBR3), and development (EGFR, DNMT1A, and FSHR) were detected in each experimental group by real-time quantitative PCR. In addition, the level of hormones in culture medium were detected using ELISA. RESULTS: Both 10(−5) M and 10(−9) M melatonin doses promoted the secretion of inhibin A and progesterone without affecting the production of inhibin B and estradiol. In addition, both promoted the gene expression of INHA, StAR, RUNX2, TGFBR3, EGFR, and DNMT1A, and inhibited the expression of BMP6, INHBB, CYP11A1, CYP19A1, and FSHR. When combined with different doses of 4-P-PDOT and luzindole, they exhibited different effects on the secretion of inhibin B, estradiol, inhibin A, and progesterone, and the expression of CYP19A1, RUNX2, BMP6, INHBB, EGFR, and DNMT1A induced by melatonin. CONCLUSION: High and low dose melatonin receptor antagonists exhibited different effects in regulating hormone secretion and the expression of various genes in response to melatonin. Therefore, concentration effects must be considered when using luzindole or 4-P-PDOT. PeerJ Inc. 2023-01-16 /pmc/articles/PMC9851050/ /pubmed/36684672 http://dx.doi.org/10.7717/peerj.14612 Text en ©2023 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Liu, Wenju
Chen, Zhihao
Li, Rui
Zheng, Menghao
Pang, Xunsheng
Wen, Aiyou
Yang, Bing
Wang, Shujuan
High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title_full High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title_fullStr High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title_full_unstemmed High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title_short High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin
title_sort high and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-p-pdot) reverse bovine granulosa cell response to melatonin
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9851050/
https://www.ncbi.nlm.nih.gov/pubmed/36684672
http://dx.doi.org/10.7717/peerj.14612
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